ArticleThe EMBO journal1998
Identification of a sequence element immediately upstream of the polypurine tract that is essential for replication of simian immunodeficiency virus.
Article in The EMBO journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Non-integrase mechanisms for dolutegravir resistance.Retrovirology · 2026Review
- HIV-1 3΄ polypurine tract mutations and integrase inhibitor resistance.AIDS (London, England) · 2025Review
- A high-quality reference genome for the fission yeast Schizosaccharomyces osmophilus.G3 (Bethesda, Md.) · 2023Article
- SiRNA-induced mutation in HIV-1 polypurine tract region and its influence on viral fitness.PloS one · 2015Article
- The effects of alternate polypurine tracts (PPTs) and mutations of sequences adjacent to the PPT on viral replication and cleavage specificity of the Rous sarcoma virus reverse transcriptase.Journal of virology · 2008Article
- High-resolution NMR analysis of the conformations of native and base analog substituted retroviral and LTR-retrotransposon PPT primers.Chemistry & biology · 2008Article
- Construction of a doxycycline-dependent simian immunodeficiency virus reveals a nontranscriptional function of tat in viral replication.Journal of virology · 2007Article
- The sequence of the RNA primer and the DNA template influence the initiation of plus-strand DNA synthesis in hepatitis B virus.Journal of molecular biology · 2007Article
- Human immunodeficiency virus bearing a disrupted central DNA flap is pathogenic in vivo.Journal of virology · 2007Review
- Purine analog substitution of the HIV-1 polypurine tract primer defines regions controlling initiation of plus-strand DNA synthesis.Nucleic acids research · 2007Article
- Importance of the N-distal AP-2 binding element in Nef for simian immunodeficiency virus replication and pathogenicity in rhesus macaques.Journal of virology · 2006Article
- Alternate polypurine tracts (PPTs) affect the rous sarcoma virus RNase H cleavage specificity and reveal a preferential cleavage following a GA dinucleotide sequence at the PPT-U3 junction.Journal of virology · 2005Article
- Cross-packaging of human immunodeficiency virus type 1 vector RNA by spleen necrosis virus proteins: construction of a new generation of spleen necrosis virus-derived retroviral vectors.Journal of virology · 2004Article
- Mutations in the 5' end of the human immunodeficiency virus type 1 polypurine tract affect RNase H cleavage specificity and virus titer.Journal of virology · 2003Article
- Hydrolysis of RNA/DNA hybrids containing nonpolar pyrimidine isosteres defines regions essential for HIV type 1 polypurine tract selection.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Wild-type levels of nuclear localization and human immunodeficiency virus type 1 replication in the absence of the central DNA flap.Journal of virology · 2002Article
- A nucleotide substitution in the tRNA(Lys) primer binding site dramatically increases replication of recombinant simian immunodeficiency virus containing a human immunodeficiency virus type 1 reverse transcriptase.Journal of virology · 2002Article
- Identification of a central DNA flap in feline immunodeficiency virus.Journal of virology · 2001Article
- Simian immunodeficiency virus in which nef and U3 sequences do not overlap replicates efficiently in vitro and in vivo in rhesus macaques.Journal of virology · 2001Article
- Persistence of pathogenic challenge virus in macaques protected by simian immunodeficiency virus SIVmacDeltanef.Journal of virology · 2001Article
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Authors and funding
2 authors.
Funding
Abstract
A short stretch of T-rich sequences immediately upstream of the polypurine tract (PPT) is highly conserved in the proviral genomes of human and simian immunodeficiency viruses (HIV and SIV). To investigate whether this 'U-box' influences SIVmac239 replication, we analyzed the properties of mutants with changes in this region of the viral genome. All mutants were either retarded in their growth (up to one month delay) or did not replicate detectably in CEMx174 cells. When U-box mutants did replicate detectably, compensatory changes were consistently observed in the viral genome. The most common compensatory change was the acquisition of thymidines immediately upstream of the PPT, but marked expansion in the length of the PPT was also observed. U-box mutants produced transiently by transfection were severely impaired in their ability to produce reverse transcripts in infectivity assays. Analysis of transiently produced mutant virus revealed no defect in RNA packaging or virus assembly. These results identify a new structural element important for an early step in the viral life cycle that includes reverse transcription.
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