ArticleThe EMBO journal1998
The ubiquitin-conjugating enzyme Pex4p of Hansenula polymorpha is required for efficient functioning of the PTS1 import machinery.
Article in The EMBO journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 97 citations in OpenAlex.
- Fusarium verticillioides Pex7/20 mediates peroxisomal PTS2 pathway import, pathogenicity, and fumonisin B1 biosynthesis.Applied microbiology and biotechnology · 2022Article
- Ubiquitin-conjugating activity by PEX4 is required for efficient protein transport to peroxisomes in Arabidopsis thaliana.The Journal of biological chemistry · 2022Article
- FgPEX4 is involved in development, pathogenicity, and cell wall integrity in Fusarium graminearum.Current genetics · 2019Article
- Elevated growth temperature decreases levels of the PEX5 peroxisome-targeting signal receptor and ameliorates defects of Arabidopsis mutants with an impaired PEX4 ubiquitin-conjugating enzyme.BMC plant biology · 2015Article
- The Pex1/Pex6 complex is a heterohexameric AAA+ motor with alternating and highly coordinated subunits.Journal of molecular biology · 2015Article
- The exportomer: the peroxisomal receptor export machinery.Cellular and molecular life sciences : CMLS · 2013Review
- Lipid droplets and peroxisomes: key players in cellular lipid homeostasis or a matter of fat--store 'em up or burn 'em down.Genetics · 2013Review
- Peroxisome-associated matrix protein degradation in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Ubiquitin signals autophagic degradation of cytosolic proteins and peroxisomes.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Characterization of glycosomal RING finger proteins of trypanosomatids.Experimental parasitology · 2007Article
- Ubiquitination of the peroxisomal import receptor Pex5p is required for its recycling.The Journal of cell biology · 2007Article
- Dynamics of the peroxisomal import cycle of PpPex20p: ubiquitin-dependent localization and regulation.The Journal of cell biology · 2006Article
- Identification and functional characterization of Arabidopsis PEROXIN4 and the interacting protein PEROXIN22.The Plant cell · 2005Article
- Ubiquitination of the peroxisomal import receptor Pex5p.The Biochemical journal · 2004Article
- Article
- Opinion: peroxisomal-protein import: is it really that complex?Nature reviews. Molecular cell biology · 2002Review
- Saccharomyces cerevisiae PTS1 receptor Pex5p interacts with the SH3 domain of the peroxisomal membrane protein Pex13p in an unconventional, non-PXXP-related manner.Molecular biology of the cell · 2000Article
- The peroxisome biogenesis factors pex4p, pex22p, pex1p, and pex6p act in the terminal steps of peroxisomal matrix protein import.Molecular and cellular biology · 2000Article
- Saccharomyces cerevisiae pex3p and pex19p are required for proper localization and stability of peroxisomal membrane proteins.The EMBO journal · 2000Article
- PEX12 interacts with PEX5 and PEX10 and acts downstream of receptor docking in peroxisomal matrix protein import.The Journal of cell biology · 1999Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have cloned the Hansenula polymorpha PEX4 gene by functional complementation of a peroxisome-deficient mutant. The PEX4 translation product, Pex4p, is a member of the ubiquitin-conjugating enzyme family. In H.polymorpha, Pex4p is a constitutive, low abundance protein. Both the original mutant and the pex4 deletion strain (Deltapex4) showed a specific defect in import of peroxisomal matrix proteins containing a C-terminal targeting signal (PTS1) and of malate synthase, whose targeting signal is not yet known. Import of the PTS2 protein amine oxidase and the insertion of the peroxisomal membrane proteins Pex3p and Pex14p was not disturbed in Deltapex4 cells. The PTS1 protein import defect in Deltapex4 cells could be suppressed by overproduction of the PTS1 receptor, Pex5p, in a dose-response related manner. In such cells, Pex5p is localized in the cytosol and in peroxisomes. The peroxisome-bound Pex5p specifically accumulated at the inner surface of the peroxisomal membrane and thus differed from Pex5p in wild-type peroxisomes, which is localized throughout the matrix. We hypothesize that in H. polymorpha Pex4p plays an essential role for normal functioning of Pex5p, possibly in mediating recycling of Pex5p from the peroxisome to the cytosol.
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