ArticleThe EMBO journal1998
Fizzy is required for activation of the APC/cyclosome in Xenopus egg extracts.
Article in The EMBO journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
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Who cites it
84 citing papers in PubMed, 194 citations in OpenAlex.
- Greatwall depletion from Xenopus oocytes reveals a key role of the cyclin B/CDK1-PP2A-B55 balance in the coordination of meiotic events.Nature communications · 2026Article
- CDC20: a novel therapeutic target in cancer.American journal of translational research · 2023Review
- Regulation of Cell Cycle Entry and Exit: A Single Cell Perspective.Comprehensive Physiology · 2019Review
- Visualizing the complex functions and mechanisms of the anaphase promoting complex/cyclosome (APC/C).Open biology · 2017Review
- APC/C and retinoblastoma interaction: cross-talk of retinoblastoma protein with the ubiquitin proteasome pathway.Bioscience reports · 2016Review
- Bub3 promotes Cdc20-dependent activation of the APC/C in S. cerevisiae.The Journal of cell biology · 2015Article
- Calcium signaling and meiotic exit at fertilization in Xenopus egg.International journal of molecular sciences · 2014Review
- The Drosophila MCPH1-B isoform is a substrate of the APCCdh1 E3 ubiquitin ligase complex.Biology open · 2014Article
- Co-regulation proteomics reveals substrates and mechanisms of APC/C-dependent degradation.The EMBO journal · 2014Article
- Changes in oscillatory dynamics in the cell cycle of early Xenopus laevis embryos.PLoS biology · 2014Article
- Sequestration of CDH1 by MAD2L2 prevents premature APC/C activation prior to anaphase onset.The Journal of cell biology · 2013Article
- The phosphatase-transcription activator EYA1 is targeted by anaphase-promoting complex/Cdh1 for degradation at M-to-G1 transition.Molecular and cellular biology · 2013Article
- Gas2l3, a novel constriction site-associated protein whose regulation is mediated by the APC/C Cdh1 complex.PloS one · 2013Article
- Studying mitotic checkpoint by illustrating dynamic kinetochore protein behavior and chromosome motion in living Drosophila syncytial embryos.Journal of visualized experiments : JoVE · 2012Article
- Structural insights into anaphase-promoting complex function and mechanism.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2011Review
- emb-1 encodes the APC16 subunit of the Caenorhabditis elegans anaphase-promoting complex.Genetics · 2011Article
- Characterization of the mechanisms controlling Greatwall activity.Molecular and cellular biology · 2011Article
- Non-proteolytic ubiquitylation counteracts the APC/C-inhibitory function of XErp1.EMBO reports · 2011Article
- Isolation and characterization of genes functionally involved in ovarian development of the giant tiger shrimp Penaeus monodon by suppression subtractive hybridization (SSH).Genetics and molecular biology · 2010Article
- APC/C-Cdh1: from cell cycle to cellular differentiation and genomic integrity.Cell cycle (Georgetown, Tex.) · 2010Review
24 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Xenopus homologue of Drosophila Fizzy and budding yeast CDC20 has been characterized. The encoded protein (X-FZY) is a component of a high molecular weight complex distinct from the APC/cyclosome. Antibodies directed against FZY were produced and shown to prevent calmodulin-dependent protein kinase II (CaMKII) from inducing the metaphase to anaphase transition of spindles assembled in vitro in Xenopus egg extracts, and this was associated with suppression of the degradation of mitotic cyclins. The same antibodies suppressed M phase-promoting factor (MPF)-dependent activation of the APC/cyclosome in interphase egg extracts, although they did not appear to alter the pattern or extent of MPF-dependent phosphorylation of APC/cyclosome subunits. As these phosphorylations are thought to be essential for APC/cyclosome activation in eggs and early embryos, we conclude that at least two events are required for MPF to activate the APC/cyclosome, allowing both chromatid segregation and full degradation of mitotic cyclins. The first one, which does not require FZY function, is the phosphorylation of APC/cyclosome subunits. The second one, that requires FZY function (even in the absence of MAD2 protein and when the spindle assembly checkpoint is not activated) is not yet understood at its molecular level.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.