ArticleBiophysical journal1998
Scanning force microscopy of DNA molecules elongated by convective fluid flow in an evaporating droplet.
Article in Biophysical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed.
- Probing the effect of PEG-DNA interactions and buffer viscosity on tethered DNA in shear flow.bioRxiv : the preprint server for biology · 2025Article
- Probing the effect of PEG-DNA interactions and buffer viscosity on tethered DNA in shear flow.PloS one · 2025Article
- Article
- Self-organized patterning through the dynamic segregation of DNA and silica nanoparticles.Scientific reports · 2014Article
- Beyond gel electrophoresis: microfluidic separations, fluorescence burst analysis, and DNA stretching.Chemical reviews · 2013Review
- Rapid generation of spatially and temporally controllable long-range concentration gradients in a microfluidic device.Lab on a chip · 2009Article
- Imaging and analysis of transcription on large, surface-mounted single template DNA molecules.Analytical biochemistry · 2008Article
- A comparative analysis of Dmc1 and Rad51 nucleoprotein filaments.Nucleic acids research · 2008Article
- A simple DNA stretching method for fluorescence imaging of single DNA molecules.Nucleic acids research · 2006Article
- Behavior of DNA fibers stretched by precise meniscus motion control.Nucleic acids research · 2001Article
- Shear-induced assembly of lambda-phage DNA.Biophysical journal · 2000Article
- Combining atomic force and fluorescence microscopy for analysis of quantum-dot labeled protein-DNA complexes.Journal of molecular recognition : JMRArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Scanning force microscopy (SFM) was used to image intact, nearly fully elongated lambda bacteriophage DNA molecules, fixed onto freshly cleaved mica surfaces. Molecular elongation and fixation were accomplished using a newly characterized fixation technique, termed "fluid fixation." Here convective fluid flows generated within an evaporating droplet of DNA solution efficiently elongate DNA molecules for fixation onto suitably charged surfaces. SFM images of a very large bacteriophage genome, G, showed the presence of double-stranded bubbles. We speculate that these structures may contain putative replication forks. Overall, the experiments presented here demonstrate the viability of using fluid fixation for the preparation of DNA molecules for SFM imaging. The combination of largely automatable optically based techniques with the high-resolution SFM imaging presented here will likely produce a high-throughput system for detailed physical mapping of genomic DNA or clones.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.