ArticleBiophysical journal1998
Capacitance flickers and pseudoflickers of small granules, measured in the cell-attached configuration.
Article in Biophysical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 49 citations in OpenAlex.
- Individual synaptic vesicles mediate stimulated exocytosis from cochlear inner hair cells.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Toward a unified picture of the exocytotic fusion pore.FEBS letters · 2018Review
- The fusion pore, 60 years after the first cartoon.FEBS letters · 2018Review
- How intravesicular composition affects exocytosis.Pflugers Archiv : European journal of physiology · 2018Review
- Fusion pores and their control of neurotransmitter and hormone release.The Journal of general physiology · 2017Review
- Kiss-and-Run Is a Significant Contributor to Synaptic Exocytosis and Endocytosis in Photoreceptors.Frontiers in cellular neuroscience · 2017Article
- Local electrostatic interactions determine the diameter of fusion pores.Channels (Austin, Tex.) · 2015Article
- Methods for cell-attached capacitance measurements in mouse adrenal chromaffin cell.Journal of visualized experiments : JoVE · 2014Article
- The plasma membrane proton pump PMA-1 is incorporated into distal parts of the hyphae independently of the Spitzenkörper in Neurospora crassa.Eukaryotic cell · 2013Article
- Intravesicular factors controlling exocytosis in chromaffin cells.Cellular and molecular neurobiology · 2010Article
- The role of the C terminus of the SNARE protein SNAP-25 in fusion pore opening and a model for fusion pore mechanics.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Hypotonicity and peptide discharge from a single vesicle.American journal of physiology. Cell physiology · 2008Article
- The fusion pores of Ca2+ -triggered exocytosis.Nature structural & molecular biology · 2008Review
- Mechanosensitive channel properties and membrane mechanics in mouse dystrophic myotubes.The Journal of physiology · 2007Article
- Shape bistability of a membrane neck: a toggle switch to control vesicle content release.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Fusion pore modulation as a presynaptic mechanism contributing to expression of long-term potentiation.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2003Article
- Silent synapses in the developing hippocampus: lack of functional AMPA receptors or low probability of glutamate release?Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Resolution of patch capacitance recordings and of fusion pore conductances in small vesicles.Biophysical journal · 2000Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have studied exocytosis of single small granules from human neutrophils by capacitance recordings in the cell-attached configuration. We found that 2.2% of the exocytotic events were flickers. The flickers always ended with a downward step. This indicates closing of the fusion pore. During flickering, the fusion pore conductance remained below 1 nS, and no net membrane transfer was detectable. After fusion pore expansion beyond 1 nS the pore expanded irreversibly, leading to rapid full incorporation of the granule/vesicle into the plasma membrane. Following exocytosis of single granules, a capacitance decrease directly related to the preceding increase was observed in 7% of the exocytotic events. This decrease followed immediately after irreversible pore expansion, and is presumably triggered by full incorporation of the vesicle into the patch membrane. The capacitance decrease could be interpreted as endocytosis triggered by exocytosis. However, the gradual decrease could also reflect a decrease in the "free" patch area following incorporation of an exocytosed vesicle. We conclude that non-stepwise capacitance changes must be interpreted with caution, since a number of factors go into determining cell or patch admittance.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.