ArticleJournal of bacteriology1998
Mutagenesis and expression of amo, which codes for ammonia monooxygenase in Nitrosomonas europaea.
Article in Journal of bacteriology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed, 65 citations in OpenAlex.
- Ammonia monooxygenase: a work in progress.Chemical science · 2026Review
- Establishing a co-culture aggregate of N-cycle bacteria to elucidate flocculation in biological wastewater treatment.Applied microbiology and biotechnology · 2025Article
- Article
- Phylogenomic analysis demonstrates a pattern of rare and long-lasting concerted evolution in prokaryotes.Communications biology · 2018Article
- Activity-Based Protein Profiling of Ammonia Monooxygenase in Nitrosomonas europaea.Applied and environmental microbiology · 2016Article
- The divergent AmoC3 subunit of ammonia monooxygenase functions as part of a stress response system in Nitrosomonas europaea.Journal of bacteriology · 2012Article
- Strategies of Nitrosomonas europaea 19718 to counter low dissolved oxygen and high nitrite concentrations.BMC microbiology · 2010Article
- Complete genome sequence of Nitrosospira multiformis, an ammonia-oxidizing bacterium from the soil environment.Applied and environmental microbiology · 2008Article
- Transcription of all amoC copies is associated with recovery of Nitrosomonas europaea from ammonia starvation.Journal of bacteriology · 2007Article
- Bacterial community shift along a subsurface geothermal water stream in a Japanese gold mine.Extremophiles : life under extreme conditions · 2005Article
- Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea.Journal of bacteriology · 2003Article
- Expression of duplicate msa genes in the salmonid pathogen Renibacterium salmoninarum.Applied and environmental microbiology · 2002Article
- Transcript analysis of multiple copies of amo (encoding ammonia monooxygenase) and hao (encoding hydroxylamine oxidoreductase) in Nitrosomonas europaea.Journal of bacteriology · 2001Article
- Molecular analysis of the pmo (particulate methane monooxygenase) operons from two type II methanotrophs.Applied and environmental microbiology · 2000Article
- Physical map location of the multicopy genes coding for ammonia monooxygenase and hydroxylamine oxidoreductase in the ammonia-oxidizing bacterium Nitrosomonas sp. strain ENI-11.Journal of bacteriology · 2000Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nitrosomonas europaea has two copies of the operon encoding ammonia monooxygenase (AMO). The nucleotide sequences of the two copies of amoA were obtained, and they were found to differ by one nucleotide. To determine if both copies of amoA were functional, insertional mutagenesis was performed to inactivate either copy of amoA alone. A DNA cassette containing the lacZ and kan genes inserted into amoA was constructed. Mutagenesis was done by using transformation and homologous recombination to mobilize the cassette into the chromosomal copies of amoA. Mutations were obtained in both copies of amoA. Either copy of amoA was sufficient to support growth when the other copy was disrupted. However, inactivation of one copy of amoA, but not the other, resulted in slower growth. Measurements of ammonia-dependent O2 consumption, which depends on AMO, confirmed that the slower-growing mutant had lower activity while the faster-growing mutant had near wild-type levels of activity. Similarly, as measured by [14C]acetylene label incorporation, there was less active AMO present in the slower-growing mutant than in the faster-growing mutant or in the wild type. Northern blot analysis of transcription likewise showed that the slower-growing mutant had less full-sized AMO mRNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.