ArticleProceedings of the National Academy of Sciences of the United States of America1998
The lepidopteran transposon vector, piggyBac, mediates germ-line transformation in the Mediterranean fruit fly.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
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Who cites it
81 citing papers in PubMed, 257 citations in OpenAlex.
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- Transgene Expression and Transposition Efficiency of Two-Component Sleeping Beauty Transposon Vector Systems Utilizing Plasmid or mRNA Encoding the Transposase.Molecular biotechnology · 2023Article
- Eliminating Malaria Vectors with Precision Guided Sterile Males.bioRxiv : the preprint server for biology · 2023Article
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- CRISPR/Cas9-induced Mutation of Sex Peptide Receptor Gene Bdspr Affects Ovary, Egg Laying, and Female Fecundity in Bactrocera dorsalis (Hendel) (Diptera: Tephritidae).Journal of insect science (Online) · 2023Article
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- Article
- In toto light sheet fluorescence microscopy live imaging datasets of Ceratitis capitata embryonic development.Scientific data · 2022Article
- Transgenic Silkworms Overexpressing Relish and Expressing Drosomycin Confer Enhanced Immunity to Multiple Pathogens.Molecular biotechnology · 2022Article
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- New self-sexingFrontiers in bioengineering and biotechnology · 2022Article
- Genetic and viral approaches to record or manipulate neurons in insects.Current opinion in insect science · 2021Review
- Unpredictable recombination of PB transposon in Silkworm: a potential risk.Molecular genetics and genomics : MGG · 2021Article
- Fine gene expression regulation by minor sequence variations downstream of the polyadenylation signal.Molecular biology reports · 2021Article
21 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The piggyBac (IFP2) short inverted terminal repeat transposable element from the cabbage looper Trichoplusia ni was tested for gene transfer vector function as part of a bipartite vector-helper system in the Mediterranean fruit fly Ceratitis capitata. A piggyBac vector marked with the medfly white gene was tested with a normally regulated piggyBac transposase helper at two different concentrations in a white eye host strain. Both experiments yielded transformants at an approximate frequency of 3-5%, with a total of six lines isolated having pigmented eyes with various levels of coloration. G1 transformant siblings from each line shared at least one common integration, with several sublines having an additional second integration. For the first transformant line isolated, two integrations were determined to be stable for 15 generations. For five of the lines, a piggyBac-mediated transposition was verified by sequencing the insertion site junctions isolated by inverse PCR that identified a characteristic piggyBac TTAA target site duplication. The efficient and stable transformation of the medfly with a lepidopteran vector represents transposon function over a relatively large evolutionary distance and suggests that the piggyBac system will be functional in a broad range of insects.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.