ArticleProceedings of the National Academy of Sciences of the United States of America1998
Transdominant genetic analysis of a growth control pathway.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 75 citations in OpenAlex.
- Target identification for small-molecule discovery in the FOXO3a tumor-suppressor pathway using a biodiverse peptide library.Cell chemical biology · 2021Article
- Lambda-display: a powerful tool for antigen discovery.Molecules (Basel, Switzerland) · 2011Review
- Selection systems based on dominant-negative transcription factors for precise genetic engineering.Nucleic acids research · 2010Article
- Dse1 may control cross talk between the pheromone and filamentation pathways in yeast.Current genetics · 2009Article
- A potentially general method for the in vivo selection of inhibitory peptides targeted at a specific protein using yeast.Current genetics · 2008Article
- The eleven-year switch of peptide aptamers.Journal of biology · 2008Review
- Identification of cell surface and secreted proteins essential for tumor cell survival using a genetic suppressor element screen.Oncogene · 2004Article
- Intracellular expression of Peptide fusions for demonstration of protein essentiality in bacteria.Antimicrobial agents and chemotherapy · 2003Article
- Genetic selection for modulators of a retinoic-acid-responsive reporter in human cells.Genetics · 2003Article
- Candida albicans Mds3p, a conserved regulator of pH responses and virulence identified through insertional mutagenesis.Genetics · 2002Article
- Characterization and use of green fluorescent proteins from Renilla mulleri and Ptilosarcus guernyi for the human cell display of functional peptides.Journal of protein chemistry · 2001Article
- Chemical genetics: ligand-based discovery of gene function.Nature reviews. Genetics · 2000Review
- Isolation of peptide aptamers that inhibit intracellular processes.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Oligonucleotide-conjugated beads for transdominant genetic experiments.Nucleic acids research · 2000Article
- Production of cyclic peptides and proteins in vivo.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- "Mutagenesis" by peptide aptamers identifies genetic network members and pathway connections.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Novel protein kinases Ark1p and Prk1p associate with and regulate the cortical actin cytoskeleton in budding yeast.The Journal of cell biology · 1999Article
- Yeast and human genes that affect the Escherichia coli SOS response.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetic selections that use proteinaceous transdominant inhibitors encoded by DNA libraries to cause mutant phenocopies may facilitate genetic analysis in traditionally nongenetic organisms. We performed a selection for random short peptides and larger protein fragments (collectively termed "perturbagens") that inhibit the yeast pheromone response pathway. Peptide and protein fragment perturbagens that permit cell division in the presence of pheromone were recovered. Two perturbagens were derived from proteins required for pheromone response, and an additional two were derived from proteins that may negatively influence the pheromone response pathway. Furthermore, three known components of the pathway were identified as probable perturbagen targets based on physical interaction assays. Thus, by selection for transdominant inhibitors of pheromone response, multiple pathway components were identified either directly as gene fragments or indirectly as the likely targets of specific perturbagens. These results, combined with the results of previous work [Holzmayer, T. A., Pestov, D. G. & Roninson, I. B. (1992) Nucl. Acids. Res. 20, 711-717; Whiteway, M., Dignard, D. & Thomas, D. Y. (1992) Proc. Natl. Acad. Sci. USA 89, 9410-9414; and Gudkov, A. V., Kazarov, A. R., Thimmapaya, R., Axenovich, S. A., Mazo, I. A. & Roninson, I. B. (1994) Proc. Natl. Acad. Sci. USA 91, 3744-3748], suggest that transdominant genetic analysis of the type described here will be broadly applicable.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.