ArticleProceedings of the National Academy of Sciences of the United States of America1998
Tyrosine phosphorylation of paxillin and focal adhesion kinase by activation of muscarinic m3 receptors is dependent on integrin engagement by the extracellular matrix.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 62 citations in OpenAlex.
- Deletion of Mechanosensory β1-integrin From Bladder Smooth Muscle Results in Voiding Dysfunction and Tissue Remodeling.Function (Oxford, England) · 2022Article
- CBFrontiers in cellular neuroscience · 2020Article
- Rac activation is key to cell motility and directionality: An experimental and modelling investigation.Computational and structural biotechnology journal · 2019Article
- Paxillin phosphorylation at serine 273 and its effects on Rac, Rho and adhesion dynamics.PLoS computational biology · 2018Article
- Orphan G protein-coupled receptor GPRC5A modulates integrin β1-mediated epithelial cell adhesion.Cell adhesion & migration · 2017Article
- Chronic baclofen desensitizes GABA(B)-mediated G-protein activation and stimulates phosphorylation of kinases in mesocorticolimbic rat brain.Neuropharmacology · 2015Article
- Prediction of functional phosphorylation sites by incorporating evolutionary information.Protein & cell · 2012Article
- Integrin and GPCR Crosstalk in the Regulation of ASM Contraction Signaling in Asthma.Journal of allergy · 2012Article
- Surface expression and limited proteolysis of ADAM10 are increased by a dominant negative inhibitor of dynamin.BMC cell biology · 2011Article
- Role of integrins in angiotensin II-induced proliferation of vascular smooth muscle cells.American journal of physiology. Cell physiology · 2011Article
- Bradykinin B2 receptor interacts with integrin alpha5beta1 to transactivate epidermal growth factor receptor in kidney cells.Molecular pharmacology · 2010Article
- Coordinate regulation of estrogen-mediated fibronectin matrix assembly and epidermal growth factor receptor transactivation by the G protein-coupled receptor, GPR30.Molecular endocrinology (Baltimore, Md.) · 2009Article
- F-prostanoid receptor alters adhesion, morphology and migration of endometrial adenocarcinoma cells.Oncogene · 2008Article
- Adhesion-dependent redistribution of MAP kinase and MEK promotes muscarinic receptor-mediated signaling to the nucleus.Journal of cellular biochemistry · 2005Article
- Article
- Myelin proteolipid protein forms a complex with integrins and may participate in integrin receptor signaling in oligodendrocytes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Rapid tyrosine phosphorylation of neuronal proteins including tau and focal adhesion kinase in response to amyloid-beta peptide exposure: involvement of Src family protein kinases.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Src family kinase involvement in muscarinic receptor-induced tyrosine phosphorylation in differentiated SH-SY5Y cells.Neurochemical research · 2001Article
- Roles of two VEGF receptors, Flt-1 and KDR, in the signal transduction of VEGF effects in human vascular endothelial cells.Oncogene · 2000Article
- Integrin-mediated activation of focal adhesion kinase is required for signaling to Jun NH2-terminal kinase and progression through the G1 phase of the cell cycle.The Journal of cell biology · 1999Article
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The G protein-coupled m1 and m3 muscarinic acetylcholine receptors increase tyrosine phosphorylation of several proteins, including the focal adhesion-associated proteins paxillin and focal adhesion kinase (FAK), but the mechanism is not understood. Activation of integrins during adhesion of cells to extracellular matrix, or stimulation of quiescent cell monolayers with G protein-coupled receptor ligands including bradykinin, bombesin, endothelin, vasopressin, and lysophosphatidic acid, also induces tyrosine phosphorylation of paxillin and FAK and formation of focal adhesions. These effects are generally independent of protein kinase C but are inhibited by agents that prevent cytoskeletal assembly or block activation of the small molecular weight G protein Rho. This report demonstrates that tyrosine phosphorylation of paxillin and FAK elicited by stimulation of muscarinic m3 receptors with the acetylcholine analog carbachol is inhibited by soluble peptides containing the arginine-glycine-aspartate motif (the recognition site for integrins found in adhesion proteins such as fibronectin) but is unaffected by peptides containing the inactive sequence arginine-glycine-glutamate. Tyrosine phosphorylation elicited by carbachol, but not by cell adhesion to fibronectin, is reduced by the protein kinase C inhibitor GF 109203X. The response to carbachol is dependent on the presence of fibronectin. Moreover, immunofluorescence studies show that carbachol treatment induces formation of stress fibers and focal adhesions. These results suggest that muscarinic receptor stimulation activates integrins via a protein kinase C-dependent mechanism. The activated integrins transmit a signal into the cell's interior leading to tyrosine phosphorylation of paxillin and FAK. This represents a novel mechanism for regulation of tyrosine phosphorylation by muscarinic receptors.
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