ArticleBiophysical journal1998
Diffusion coefficients in the lateral intercellular spaces of Madin-Darby canine kidney cell epithelium determined with caged compounds.
Article in Biophysical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 45 citations in OpenAlex.
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- Photolysis of a Caged, Fast-Equilibrating Glutamate Receptor Antagonist, MNI-CagedFrontiers in cellular neuroscience · 2018Article
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- Photoactivatable, biologically-relevant phenols with sensitivity toward 2-photon excitation.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2015Article
- Role of Epidermal Growth Factor-Triggered PI3K/Akt Signaling in the Migration of Medulloblastoma-Derived Cells.Cellular and molecular bioengineering · 2012Article
- Routes of epithelial water flow: aquaporins versus cotransporters.Biophysical journal · 2010Article
- Microchannel-nanopore device for bacterial chemotaxis assays.Analytical chemistry · 2010Article
- An EGFR autocrine loop encodes a slow-reacting but dominant mode of mechanotransduction in a polarized epithelium.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2010Article
- Compartmentalization of the outer hair cell demonstrated by slow diffusion in the extracisternal space.Biophysical journal · 2009Article
- Computational modeling of extracellular mechanotransduction.Biophysical journal · 2006Article
- The invagination of excess surface area by shrinking neurons.Biophysical journal · 2003Article
- Changes in brain cell shape create residual extracellular space volume and explain tortuosity behavior during osmotic challenge.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Water does not flow across the tight junctions of MDCK cell epithelium.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The diffusion coefficients of two caged fluorescent dyes were measured in free solution and in the lateral intercellular spaces (LIS) of cultured Madin-Darby canine kidney (MDCK) cells after photoactivation by illumination with a continuous or pulsed UV laser. Both quantitative video imaging and a new photometric method were utilized to determine the rates of diffusion of the caged fluorescent dyes: 8-((4,5-dimethoxy-2-nitrobenzyl)oxy)pyrene-1,3,6-trisulfonic acid (DMNB-HPTS) and (4,5-dimethoxy-2-nitrobenzyl) fluorescein dextran (10,000 MW) (DMNB-caged fluorescein dextran). The diffusion coefficients at 37 degrees C in free solution were 3.3 x 10(-6) cm2/s (HPTS) and 0.98 x 10(-6) cm2/s (10,000 MW dextran). Diffusion of HPTS within nominally linear stretches of the LIS of MDCK cells grown on glass coverslips was indistinguishable from that in free solution, whereas dextran showed a 1.6 +/- 0.5-fold reduction in diffusivity. Measurements of HPTS diffusion within the LIS of multicellular regions also exhibited a diffusivity comparable to the free solution value. The restriction to diffusion of the dextran within the LIS may be due to molecular hindrance.
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