ArticleJournal of muscle research and cell motility1998
Mechanical alterations in smooth muscle from mice lacking desmin.
Article in Journal of muscle research and cell motility, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 77 citations in OpenAlex.
- Micro-RNA-338-3p Promotes the Development of Atherosclerosis by Targeting Desmin and Promoting Proliferation.Molecular biotechnology · 2021Article
- Increased expression of desmin and vimentin reduces bladder smooth muscle contractility via JNK2.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- An update on clonality: what smooth muscle cell type makes up the atherosclerotic plaque?F1000Research · 2018Review
- The roles and regulation of the actin cytoskeleton, intermediate filaments and microtubules in smooth muscle cell migration.Respiratory research · 2017Review
- The role of mechanotransduction on vascular smooth muscle myocytes' [corrected] cytoskeleton and contractile function.Anatomical record (Hoboken, N.J. : 2007) · 2014Review
- The small GTPase Rac1 is required for smooth muscle contraction.The Journal of physiology · 2014Article
- Knockdown of desmin in zebrafish larvae affects interfilament spacing and mechanical properties of skeletal muscle.The Journal of general physiology · 2013Article
- Intermediate filaments in smooth muscle.American journal of physiology. Cell physiology · 2008Review
- Differentiation of cardiomyocytes requires functional serine residues within the amino-terminal domain of desmin.Differentiation; research in biological diversity · 2007Article
- Desmin stimulates differentiation of cardiomyocytes and up-regulation of brachyury and nkx2.5.Differentiation; research in biological diversity · 2007Article
- Desmin filaments influence myofilament spacing and lateral compliance of slow skeletal muscle fibers.Biophysical journal · 2005Article
- Nonmuscle Myosin motor of smooth muscle.The Journal of general physiology · 2003Article
- Lower active force generation and improved fatigue resistance in skeletal muscle from desmin deficient mice.Journal of muscle research and cell motility · 2003Article
- Smooth, slow and smart muscle motors.Journal of muscle research and cell motility · 2003Review
- Decreased shortening velocity and altered myosin isoforms in guinea-pig hypertrophic intestinal smooth muscle.The Journal of physiology · 2002Article
- Mechanical function of intermediate filaments in arteries of different size examined using desmin deficient mice.The Journal of physiology · 2002Article
- Desmin cytoskeleton in healthy and failing heart.Heart failure reviews · 2000Review
- Nspl1, a new Z-band-associated protein.Journal of muscle research and cell motility · 1999Article
- A dysfunctional desmin mutation in a patient with severe generalized myopathy.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mice with a null mutation introduced in the desmin gene were used to study the mechanical role of intermediate filaments in smooth muscle cells. Vas deferens (VD), urinary bladder (UB) and portal vein (PV) preparations were obtained from adult animals lacking desmin (Des -/-) and from age- and weight-matched wild-type animals (Des +/+). Active force per cross-sectional area was decreased in the smooth muscle of the Des -/- compared with Des +/+ mice (VD to 42%; UB to 34%). Quantitative gel electrophoresis suggests a marginally lower cellular content of myosin, but the organization of the contractile apparatus appeared unchanged by electron microscopy. A similar reduction in stress was measured in Des -/- skinned fibres showing that altered activation mechanisms were not involved. The results indicate that the reduced active force is caused by low intrinsic force generation of the contractile filaments or subtle modifications in the coupling between the contractile elements and the cytoskeleton. The relationship between length and passive stress was less steep in the Des -/- samples and a second length force curve after maximal extension revealed a loss of passive stress. The maximal shortening velocity was reduced in Des -/- skinned VD and UB preparations by approximately 25-40%. This was associated with an increased relative content of the basic essential myosin light chain, suggesting that alterations in the contractile system towards a slower, more economical muscle had occurred. PV preparations showed no difference in mechanical properties in Des +/+ and Des -/- animals, a result that was consistent with the predominance of vimentin instead of desmin in this vascular tissue. In conclusion, the results show that, although intermediate filaments in smooth muscle are not required for force generation or maintenance of passive tension, they have a role in cellular transmission of both active and passive force.
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