ArticleThe Plant cell1998
Initiation and maintenance of virus-induced gene silencing
Article in The Plant cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 346 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
346 citing papers in PubMed, 989 citations in OpenAlex.
- The dehydrin protein COR15 enhances antiviral RNA silencing by preventing viral protein-mediated SGS3 degradation and promoting SGS3 condensate formation.Plant communications · 2026Article
- Construction and Characterization of an Attenuated Vaccine Vector Based on Cucumber Mosaic Virus RNA2.Life (Basel, Switzerland) · 2026Article
- Thermotherapy Treatments of Fruit Trees of the Genus Prunus Infected by Viruses and Bacteria.Plants (Basel, Switzerland) · 2026Article
- Targeted disruption of vacuolar-sorting protein and inhibitor of apoptosis genes via virus-induced gene silencing alters survival and reproduction of Diaphorina citri.Pest management science · 2026Article
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- Methionine Synthase Positively Regulates Plant Defence to Both RNA and DNA Viruses and Is Useful for Developing Broad-Spectrum Antiviral Resistance in Crops.Plant biotechnology journal · 2026Article
- Red clover RNA virus 1: functional analysis of ORF2 protein, associations with co-infecting viruses, and seed transmission.Archives of virology · 2026Article
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- Causes and consequences of experimental variation in Nicotiana benthamiana transient expression.Nature communications · 2026Article
- Nuclear coat protein drives a multifaceted tolerance program during Turnip crinkle virus infection.Research square · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Gene Inactivation in Transgenic Plants-A Unique Model for Studying Epigenetic Regulation of Gene Expression.Plants (Basel, Switzerland) · 2026Review
- Virus-induced gene silencing as a tool for functional genomics in weeds: Challenges and future directions.Genetics and molecular biology · 2026Article
- Review
- Viral vectors for antimicrobial peptide expression: a new path for crop protection.Frontiers in microbiology · 2026Review
- APathogens (Basel, Switzerland) · 2025Article
- Truncated CMV2bPlant methods · 2025Article
- Nanoparticles and Nanocarriers for Managing Plant Viral Diseases.Plants (Basel, Switzerland) · 2025Review
- Article
- Seeing is believing: Genome editing made easy with RUBY for educational purposes.Plant physiology · 2025Article
286 more citing papers are in PubMed but not listed here.
Corrections and comments
- Correction · 2015-07-01Duplication of/in Image · Error in Image · Manipulation of Images · · Olivier Voinnet has finding of scientific misconduct http://www2.cnrs.fr/en/2593.htm
- Erratum issuedCorrection.2015
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The phytoene desaturase (PDS) gene of Nicotiana benthamiana was silenced in plants infected with potato virus X (PVX) vectors carrying PDS inserts, and a green fluorescent protein (GFP) transgene was silenced in plants infected with PVX-GFP. This virus-induced gene silencing (VIGS) is post-transcriptional and cytoplasmic because it is targeted against exons rather than introns of PDS RNA and against viral RNAs. Although PDS and GFP RNAs are most likely targeted through the same mechanism, the VIGS phenotypes differed in two respects. PDS mRNA was targeted by VIGS in all green tissue of the PVX-PDS-infected plant, whereas PVX-PDS was not affected. In contrast, VIGS of the GFP was targeted against PVX-GFP. Initially, VIGS of the GFP was initiated in all green tissues, as occurred with PDS VIGS. However, after 30 days of infection, the GFP VIGS was no longer initiated in newly emerging leaves, although it was maintained in tissue in which it had already been initiated. Based on these analyses, we propose a model for VIGS in which the initiation of VIGS is dependent on the virus and maintenance of it is virus independent.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.