ArticleMolecular and cellular biology1998
Targeting to transcriptionally active loci by the hydrophilic N-terminal domain of Drosophila DNA topoisomerase I.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 48 citations in OpenAlex.
- Exploration of the Role of the C-Terminal Domain of Human DNA Topoisomerase IIα in Catalytic Activity.ACS omega · 2021Article
- Dynamic localization of DNA topoisomerase I and its functional relevance during Drosophila development.G3 (Bethesda, Md.) · 2021Article
- When DNA Topology Turns Deadly - RNA Polymerases Dig in Their R-Loops to Stand Their Ground: New Positive and Negative (Super)Twists in the Replication-Transcription Conflict.Trends in genetics : TIG · 2018Review
- DNA and RNA topoisomerase activities of Top3β are promoted by mediator protein Tudor domain-containing protein 3.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- The identification of putative RNA polymerase II C-terminal domain associated proteins in red and green algae.Transcription · 2014Article
- Top3α is required during the convergent migration step of double Holliday junction dissolution.PloS one · 2014Article
- Negative regulation of mitochondrial transcription by mitochondrial topoisomerase I.Nucleic acids research · 2013Article
- Topoisomerase IIβ deficiency enhances camptothecin-induced apoptosis.The Journal of biological chemistry · 2013Article
- HJURP is a CENP-A chromatin assembly factor sufficient to form a functional de novo kinetochore.The Journal of cell biology · 2011Article
- The SR protein B52/SRp55 is required for DNA topoisomerase I recruitment to chromatin, mRNA release and transcription shutdown.PLoS genetics · 2010Article
- The phosphoCTD-interacting domain of Topoisomerase I.Biochemical and biophysical research communications · 2010Article
- Biochemical analyses of transcriptional regulatory mechanisms in a chromatin context.Methods (San Diego, Calif.) · 2007Article
- Topoisomerase IIIalpha and Bloom's helicase can resolve a mobile double Holliday junction substrate through convergent branch migration.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Disruption of a DNA topoisomerase I gene affects morphogenesis in Arabidopsis.The Plant cell · 2002Article
- Preferential cleavage of plasmid-based R-loops and D-loops by Drosophila topoisomerase IIIbeta.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Cellular roles of DNA topoisomerases: a molecular perspective.Nature reviews. Molecular cell biology · 2002Review
- Subnuclear distribution of topoisomerase I is linked to ongoing transcription and p53 status.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Characterization of BTBD1 and BTBD2, two similar BTB-domain-containing Kelch-like proteins that interact with Topoisomerase I.BMC genomics · 2002Article
- Transcriptional consequences of topoisomerase inhibition.Molecular and cellular biology · 2001Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
DNA topoisomerase I (topo I) from Drosophila melanogaster contains a nonconserved, hydrophilic N-terminal domain of about 430 residues upstream of the conserved core domains. Deletion of this N terminus did not affect the catalytic activity of topo I, while further removal of sequences into the conserved regions inactivated its enzymatic activity. We have investigated the cellular function of the Drosophila topo I N-terminal domain with top1-lacZ transgenes. There was at least one putative nuclear localization signal within the first 315 residues of the N-terminal domain that allows efficient import of the large chimeric proteins into Drosophila nuclei. The top1-lacZ fusion proteins colocalized with RNA polymerase II (pol II) at developmental puffs on the polytene chromosomes. Either topo I or the top1-lacZ fusion protein was colocalized with RNA pol II in some but not all of the nonpuff, interband loci. However, the fusion proteins as well as RNA pol II were recruited to heat shock puffs during heat treatment, and they returned to the developmental puffs after recovery from heat shock. By immunoprecipitation, we showed that two of the largest subunits of RNA pol II coprecipitated with the N-terminal 315-residue fusion protein by using antibodies against beta-galactosidase. These data suggest that the topo I fusion protein can be localized to the transcriptional complex on chromatin and that the N-terminal 315 residues were sufficient to respond to cellular processes, especially during the reprogramming of gene expression.
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