ArticleMolecular and cellular biology1998
DNA-dependent protein kinase phosphorylation of IkappaB alpha and IkappaB beta regulates NF-kappaB DNA binding properties.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 72 citations in OpenAlex.
- Induction of APOBEC3B expression by chemotherapy drugs is mediated by DNA-PK-directed activation of NF-κB.Oncogene · 2021Article
- DNA-PKcs inhibition impairs HDAC6-mediated HSP90 chaperone function on Aurora A and enhances HDACs inhibitor-induced cell killing by increasing mitotic aberrant spindle assembly.Cell cycle (Georgetown, Tex.) · 2021Article
- Review
- The role of DNA-PK in aging and energy metabolism.The FEBS journal · 2018Review
- Engagement of Components of DNA-Break Repair Complex and NFκB in Hsp70A1A Transcription Upregulation by Heat Shock.PloS one · 2017Article
- β-Carotene-induced apoptosis is mediated with loss of Ku proteins in gastric cancer AGS cells.Genes & nutrition · 2015Article
- Cellular stress response and innate immune signaling: integrating pathways in host defense and inflammation.Journal of leukocyte biology · 2013Review
- Lymphocyte development: integration of DNA damage response signaling.Advances in immunology · 2012Review
- Phosphorylation of p50 NF-kappaB at a single serine residue by DNA-dependent protein kinase is critical for VCAM-1 expression upon TNF treatment.The Journal of biological chemistry · 2010Article
- CK2 phosphorylation of Pdx-1 regulates its transcription factor activity.Cellular and molecular life sciences : CMLS · 2010Article
- Progesterone receptor inhibits aromatase and inflammatory response pathways in breast cancer cells via ligand-dependent and ligand-independent mechanisms.Molecular endocrinology (Baltimore, Md.) · 2008Article
- Article
- Differential gene expression in human glioma cells: correlation with presence or absence of DNA-dependent protein kinase.Gene expression · 2003Article
- Human autoimmune sera as molecular probes for the identification of an autoantigen kinase signaling pathway.The Journal of experimental medicine · 2002Article
- CD40 activation in epithelial ovarian carcinoma cells modulates growth, apoptosis, and cytokine secretion.Molecular pathology : MP · 2002Article
- Transfection of single-stranded hepatitis A virus RNA activates MHC class I pathway.Clinical and experimental immunology · 2002Article
- Increased and correlated nuclear factor-kappa B and Ku autoantigen activities are associated with development of multidrug resistance.Oncogene · 2001Article
- New insights into the role of nuclear factor-kappaB in cell growth regulation.The American journal of pathology · 2001Review
- Nuclear clusterin/XIP8, an x-ray-induced Ku70-binding protein that signals cell death.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Inactivation of p53 by human T-cell lymphotropic virus type 1 Tax requires activation of the NF-kappaB pathway and is dependent on p53 phosphorylation.Molecular and cellular biology · 2000Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Regulation of the IkappaB alpha and IkappaB beta proteins is critical for modulating NF-kappaB-directed gene expression. Both IkappaB alpha and IkappaB beta are substrates for cellular kinases that phosphorylate the amino and carboxy termini of these proteins and regulate their function. In this study, we utilized a biochemical fractionation scheme to purify a kinase activity which phosphorylates residues in the amino and carboxy termini of both IkappaB alpha and IkappaB beta. Peptide microsequence analysis by capillary high-performance liquid chromatography ion trap mass spectroscopy revealed that this kinase was the DNA-dependent protein kinase catalytic subunit (DNA-PKcs). DNA-PK phosphorylates serine residue 36 but not serine residue 32 in the amino terminus of IkappaB alpha and also phosphorylates threonine residue 273 in the carboxy terminus of this protein. To determine the biological relevance of DNA-PK phosphorylation of IkappaB alpha, murine severe combined immunodeficiency (SCID) cell lines which lack the DNA-PKcs gene were analyzed. Gel retardation analysis using extract prepared from these cells demonstrated constitutive nuclear NF-kappaB DNA binding activity, which was not detected in extracts prepared from SCID cells complemented with the human DNA-PKcs gene. Furthermore, IkappaB alpha that was phosphorylated by DNA-PK was a more potent inhibitor of NF-kappaB binding than nonphosphorylated IkappaB alpha. These results suggest that DNA-PK phosphorylation of IkappaB alpha increases its interaction with NF-kappaB to reduce NF-kappaB DNA binding properties.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.