ArticleMolecular and cellular biology1998
Molecular mechanism governing heme signaling in yeast: a higher-order complex mediates heme regulation of the transcriptional activator HAP1.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 110 citations in OpenAlex.
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- GUN control in retrograde signaling: How GENOMES UNCOUPLED proteins adjust nuclear gene expression to plastid biogenesis.The Plant cell · 2021Review
- Proteomic analysis of haem-binding protein fromPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Mitochondrial-nuclear heme trafficking in budding yeast is regulated by GTPases that control mitochondrial dynamics and ER contact sites.Journal of cell science · 2020Article
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- Heme bioavailability and signaling in response to stress in yeast cells.The Journal of biological chemistry · 2018Article
- Multidrug resistance in fungi: regulation of transporter-encoding gene expression.Frontiers in physiology · 2014Review
- The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation.Cell & bioscience · 2012Article
- Biology of the heat shock response and protein chaperones: budding yeast (Saccharomyces cerevisiae) as a model system.Microbiology and molecular biology reviews : MMBR · 2012Review
- Review
- Hypoxia elicits broad and systematic changes in protein subcellular localization.American journal of physiology. Cell physiology · 2011Article
- Nitric oxide and nitrosative stress tolerance in yeast.Biochemical Society transactions · 2011Article
- Regulation of hypoxia adaptation: an overlooked virulence attribute of pathogenic fungi?Medical mycology · 2010Review
- A predictive model of the oxygen and heme regulatory network in yeast.PLoS computational biology · 2008Article
- Oxygen-dependent transcriptional regulator Hap1p limits glucose uptake by repressing the expression of the major glucose transporter gene RAG1 in Kluyveromyces lactis.Eukaryotic cell · 2008Article
- CTA4 transcription factor mediates induction of nitrosative stress response in Candida albicans.Eukaryotic cell · 2008Article
- Regulation of the HAP1 gene involves positive actions of histone deacetylases.Biochemical and biophysical research communications · 2007Article
- Characterization and prediction of protein-protein interactions within and between complexes.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Identification of a plastid response element that acts as an enhancer within the Chlamydomonas HSP70A promoter.Nucleic acids research · 2006Article
- Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Apart from serving as a prosthetic group in globins and enzymes, heme is a key regulator controlling a wide range of molecular and cellular processes involved in oxygen sensing and utilization. To gain insights into molecular mechanisms of heme signaling and oxygen sensing in eukaryotes, we investigated the yeast heme-responsive transcriptional activator HAP1. HAP1 activity is regulated precisely and tightly by heme. Here we show that in the absence of heme, HAP1 forms a biochemically distinctive higher-order complex. Our data suggest that this complex contains HAP1 and four other cellular proteins including Hsp82 and Ydj1. The formation of this complex is directly correlated with HAP1 repression in the absence of heme, and mutational or heme disruption of the complex correlates with HAP1 activation, suggesting that this complex is responsible for heme regulation of HAP1 activity. Further, we determined HAP1 domains required for heme regulation: three domains-the dimerization domain, the heme domain, and the HRM7 (heme-responsive motif 7) domain-cooperate to form the higher-order complex and mediate heme regulation. Strikingly, we uncovered a novel function for the HAP1 dimerization domain: it not only allows dimerization but also provides critical functions in heme regulation and transcriptional activation. Our studies provide significant insights into the molecular events leading to heme activation of HAP1 and may shed light on molecular mechanisms of various heme-controlled biological processes in diverse organisms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.