ArticleMolecular and cellular biology1998
Even-skipped represses transcription by binding TATA binding protein and blocking the TFIID-TATA box interaction.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 56 citations in OpenAlex.
- Trimeric complexes of Antp-TBP with TFIIEβ or Exd modulate transcriptional activity.Hereditas · 2022Article
- Organization of Embryonic Morphogenesis via Mechanical Information.Developmental cell · 2019Article
- Evx1 and Evx2 specify excitatory neurotransmitter fates and suppress inhibitory fates through a Pax2-independent mechanism.Neural development · 2016Article
- Transcriptional repression of AIB1 by FoxG1 leads to apoptosis in breast cancer cells.Molecular endocrinology (Baltimore, Md.) · 2013Article
- Histone acetyltransferases in rice (Oryza sativa L.): phylogenetic analysis, subcellular localization and expression.BMC plant biology · 2012Article
- Elongation factor 1 alpha1 and genes associated with Usher syndromes are downstream targets of GBX2.PloS one · 2012Article
- A BEN-domain-containing protein associates with heterochromatin and represses transcription.Journal of cell science · 2011Article
- Induction and patterning of trunk and tail neural ectoderm by the homeobox gene eve1 in zebrafish embryos.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- The HOX homeodomain proteins block CBP histone acetyltransferase activity.Molecular and cellular biology · 2001Article
- p53 Stimulates TFIID-TFIIA-promoter complex assembly, and p53-T antigen complex inhibits TATA binding protein-TATA interaction.Molecular and cellular biology · 2001Article
- Groucho augments the repression of multiple Even skipped target genes in establishing parasegment boundaries.Development (Cambridge, England) · 2001Article
- Drosophila Mediator complex is broadly utilized by diverse gene-specific transcription factors at different types of core promoters.Molecular and cellular biology · 2001Article
- MeCP2 driven transcriptional repression in vitro: selectivity for methylated DNA, action at a distance and contacts with the basal transcription machinery.Nucleic acids research · 2000Article
- Transcriptional repression by blimp-1 (PRDI-BF1) involves recruitment of histone deacetylase.Molecular and cellular biology · 2000Article
- Article
- Alleviation of human papillomavirus E2-mediated transcriptional repression via formation of a TATA binding protein (or TFIID)-TFIIB-RNA polymerase II-TFIIF preinitiation complex.Molecular and cellular biology · 2000Article
- Identification of a portable repression domain and an E1A-responsive activation domain in Pax4: a possible role of Pax4 as a transcriptional repressor in the pancreas.Molecular and cellular biology · 1999Article
- Neural restrictive silencer factor recruits mSin3 and histone deacetylase complex to repress neuron-specific target genes.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Regulation of the Wilms' tumour suppressor protein transcriptional activation domain.Oncogene · 1999Article
- Evolutionary conserved mechanism of transcriptional repression by even-skipped.Nucleic acids research · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The Drosophila homeodomain protein Even-skipped (Eve) is a transcriptional repressor, and previous studies have suggested that it functions by interfering with the basal transcription machinery. Here we describe experiments indicating that the mechanism of Eve repression involves a direct interaction with the TATA binding protein (TBP) that blocks binding of TBP-TFIID to the promoter. We first compared Eve activities in in vitro transcription systems reconstituted with either all the general transcription factors or only TBP, TFIIB, TFIIF30, and RNA polymerase II. In each case, equivalent and very efficient levels of repression were observed, indicating that no factors other than those in the minimal system are required for repression. We then show that Eve can function efficiently when its recognition sites are far from the promoter and that the same regions of Eve required for repression in vivo are necessary and sufficient for in vitro repression. This includes, in addition to an Ala-Pro-rich region, residues within the homeodomain. Using GAL4-Eve fusion proteins, we demonstrate that the homeodomain plays a role in repression in addition to DNA binding, which is to facilitate interaction with TBP. Single-round transcription experiments indicate that Eve must function prior to TBP binding to the promoter, suggesting a mechanism whereby Eve represses by competing with the TATA box for TBP binding. Consistent with this, excess TATA box-containing oligonucleotide is shown to specifically and efficiently disrupt the TBP-Eve interaction. Importantly, we show that Eve binds directly to TFIID and that this interaction can also be disrupted by the TATA oligonucleotide. We conclude that Eve represses transcription via a direct interaction with TBP that blocks TFIID binding to the promoter.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.