ArticleMolecular and cellular biology1998
Requirement for activation of the serine-threonine kinase Akt (protein kinase B) in insulin stimulation of protein synthesis but not of glucose transport.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
65 citing papers in PubMed, 334 citations in OpenAlex.
- Increased hepatic Akt phosphorylation alleviated glucose intolerance and improved liver function in leptin-deficient mice.Clinical and experimental hepatology · 2023Article
- Low Muscle Mass in Patients Receiving Hemodialysis: Correlations with Vascular Calcification and Vascular Access Failure.Journal of clinical medicine · 2021Article
- Palmitoylethanolamide: A Natural Compound for Health Management.International journal of molecular sciences · 2021Review
- β-Adrenergic Receptor and Insulin Resistance in the Heart.Biomolecules & therapeutics · 2017Review
- mRNAs containing the histone 3' stem-loop are degraded primarily by decapping mediated by oligouridylation of the 3' end.RNA (New York, N.Y.) · 2013Article
- Circumventing cellular control of PP2A by methylation promotes transformation in an Akt-dependent manner.Neoplasia (New York, N.Y.) · 2012Article
- Attenuation of age-related changes in FOXO3a activity and the PI3K/Akt pathway by short-term feeding of ferulate.Age (Dordrecht, Netherlands) · 2012Article
- Functional and direct interaction between the RNA binding protein HuD and active Akt1.Nucleic acids research · 2012Article
- Resistance of Akt kinases to dephosphorylation through ATP-dependent conformational plasticity.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Insulin/IGF-I regulation of necdin and brown adipocyte differentiation via CREB- and FoxO1-associated pathways.Endocrinology · 2011Article
- Selective regulation of heme oxygenase-1 expression and function by insulin through IRS1/phosphoinositide 3-kinase/Akt-2 pathway.The Journal of biological chemistry · 2008Article
- Mutation of the PDK1 PH domain inhibits protein kinase B/Akt, leading to small size and insulin resistance.Molecular and cellular biology · 2008Article
- Ca2+-permeable AMPA receptors regulate growth of human glioblastoma via Akt activation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Brain tumor formation in tuberous sclerosis depends on Erk activation.Neuromolecular medicine · 2007Article
- Insulin-like growth factor-1 (IGF-1) induces the activation/phosphorylation of Akt kinase and cAMP response element-binding protein (CREB) by activating different signaling pathways in PC12 cells.BMC neuroscience · 2006Article
- The hypoglycaemic activity of fenugreek seed extract is mediated through the stimulation of an insulin signalling pathway.British journal of pharmacology · 2005Article
- PI3K rescues the detrimental effects of chronic Akt activation in the heart during ischemia/reperfusion injury.The Journal of clinical investigation · 2005Article
- Regulation of insulin signalling by hyperinsulinaemia: role of IRS-1/2 serine phosphorylation and the mTOR/p70 S6K pathway.Diabetologia · 2005Article
- Inhibition of endogenous SHIP2 ameliorates insulin resistance caused by chronic insulin treatment in 3T3-L1 adipocytes.Diabetologia · 2005Article
- PKClambda regulates glucose-induced insulin secretion through modulation of gene expression in pancreatic beta cells.The Journal of clinical investigation · 2005Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A wide variety of biological activities including the major metabolic actions of insulin is regulated by phosphatidylinositol (PI) 3-kinase. However, the downstream effectors of the various signaling pathways that emanate from PI 3-kinase remain unclear. Akt (protein kinase B), a serine-threonine kinase with a pleckstrin homology domain, is thought to be one such downstream effector. A mutant Akt (Akt-AA) in which the phosphorylation sites (Thr308 and Ser473) targeted by growth factors are replaced by alanine has now been shown to lack protein kinase activity and, when overexpressed in CHO cells or 3T3-L1 adipocytes with the use of an adenovirus vector, to inhibit insulin-induced activation of endogenous Akt. Akt-AA thus acts in a dominant negative manner in intact cells. Insulin-stimulated protein synthesis, which is sensitive to wortmannin, a pharmacological inhibitor of PI 3-kinase, was abolished by overexpression of Akt-AA without an effect on amino acid transport into the cells, suggesting that Akt is required for insulin-stimulated protein synthesis. Insulin activation of p70 S6 kinase was inhibited by approximately 75% in CHO cells and approximately 30% in 3T3-L1 adipocytes, whereas insulin-induced activation of endogenous Akt was inhibited by 80 to 95%, by expression of Akt-AA. Thus, Akt activity appears to be required, at least in part, for insulin stimulation of p70 S6 kinase. However, insulin-stimulated glucose uptake in both CHO cells and 3T3-L1 adipocytes was not affected by overexpression of Akt-AA, suggesting that Akt is not required for this effect of insulin. These data indicate that Akt acts as a downstream effector in some, but not all, of the signaling pathways downstream of PI 3-kinase.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.