ArticleNucleic acids research1998
Cap ribose methylation of c-mos mRNA stimulates translation and oocyte maturation in Xenopus laevis.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it, 98 citations in OpenAlex.
- Translational control of cell growth and malignancy by the CPEBs.Nature reviews. Cancer · 2013Pooled it
- Preclinical and Clinical Demonstration of Immunogenicity by mRNA Vaccines against H10N8 and H7N9 Influenza Viruses.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Trial
- Cap-adjacent 2'-O-ribose methylation of RNA in C. elegans is required for postembryonic growth and germline development in the presence of the decapping exonuclease EOL-1.Nucleic acids research · 2026Article
- CK2 phosphorylation of CMTR1 promotes RNA cap formation and influenza virus infection.Cell reports · 2024Article
- 2'-RNA (New York, N.Y.) · 2024Review
- CPEB and translational control by cytoplasmic polyadenylation: impact on synaptic plasticity, learning, and memory.Molecular psychiatry · 2023Review
- CMTR1 is recruited to transcription start sites and promotes ribosomal protein and histone gene expression in embryonic stem cells.Nucleic acids research · 2022Article
- The Past, Present, and Future of Non-Viral CAR T Cells.Frontiers in immunology · 2022Review
- CMTR1-Catalyzed 2'-O-Ribose Methylation Controls Neuronal Development by Regulating Camk2α Expression Independent of RIG-I Signaling.Cell reports · 2020Article
- So close, no matter how far: multiple paths connecting transcription to mRNA translation in eukaryotes.EMBO reports · 2020Review
- Optimization of an mRNA vaccine assisted with cyclodextrin-polyethyleneimine conjugates.Drug delivery and translational research · 2020Article
- The mRNA Cap 2'-mSphere · 2020Article
- mRNA Delivery for Therapeutic Anti-HER2 Antibody Expression In Vivo.Molecular therapy : the journal of the American Society of Gene Therapy · 2019Article
- mRNA cap regulation in mammalian cell function and fate.Biochimica et biophysica acta. Gene regulatory mechanisms · 2019Review
- Review
- A recap of RNA recapping.Wiley interdisciplinary reviews. RNA · 2019Review
- Human RNA cap1 methyltransferase CMTr1 cooperates with RNA helicase DHX15 to modify RNAs with highly structured 5' termini.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Article
- DHX15 regulates CMTR1-dependent gene expression and cell proliferation.Life science alliance · 2018Article
- Truncated forms of U2 snRNA (U2-tfs) are shunted toward a novel uridylylation pathway that differs from the degradation pathway for U1-tfs.RNA biology · 2018Article
- Regulation and function of CMTR1-dependent mRNA cap methylation.Wiley interdisciplinary reviews. RNA · 2017Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Xenopus oocytes, progesterone stimulates the cytoplasmic polyadenylation and resulting translational activation of c-mos mRNA, which is necessary for the induction of oocyte maturation. Although details of the biochemistry of polyadenylation are beginning to emerge, the mechanism by which 3' poly(A) addition stimulates translation initiation is enigmatic. A previous report showed that polyadenylation induced cap-specific 2'-O-methylation, and suggested that this 5' end modification was important for translational activation. Here, we demonstrate that injected c-mos RNA undergoes polyadenylation and cap ribose methylation. Inhibition of this methylation by S-isobutylthioadenosine (SIBA), a methyltransferase inhibitor, has little effect on progesterone-induced c-mos mRNA polyadenylation or general protein synthesis, but prevents the synthesis of Mos protein as well as oocyte maturation. Maturation can be rescued, however, by the injection of factors that act downstream of Mos, such as cyclin A and B mRNAs. Most importantly, we show that the translational efficiency of injected mRNAs containing cap-specific 2'-O-methylation (cap I) is significantly enhanced compared to RNAs that do not contain the methylated ribose (cap 0). These results suggest that cap ribose methylation of c-mos mRNA is important for translational recruitment and for the progression of oocytes through meiosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.