ArticleThe Journal of physiology1998
Modulation of ATP-gated non-selective cation channel (P2X1 receptor) activation and desensitization by the actin cytoskeleton.
Article in The Journal of physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 32 citations in OpenAlex.
- Microglia proliferation is controlled by P2X7 receptors in a Pannexin-1-independent manner during early embryonic spinal cord invasion.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Identification of human P2X1 receptor-interacting proteins reveals a role of the cytoskeleton in receptor regulation.The Journal of biological chemistry · 2011Article
- Colchicine inhibits cationic dye uptake induced by ATP in P2X2 and P2X7 receptor-expressing cells: implications for its therapeutic action.British journal of pharmacology · 2011Article
- Interaction of P2 purinergic receptors with cellular macromolecules.Naunyn-Schmiedeberg's archives of pharmacology · 2008Review
- ATP modulation of excitatory synapses onto interneurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Purification and Recognition of Recombinant Mouse P2X(1) Receptors Expressed in a Baculovirus System.Drug development research · 2000Article
- Inactivation of P2X2 purinoceptors by divalent cations.The Journal of physiology · 2000Article
- Evidence for the involvement of spinal endogenous ATP and P2X receptors in nociceptive responses caused by formalin and capsaicin in mice.British journal of pharmacology · 1999Article
- Identification of amino acids within the P2X2 receptor C-terminus that regulate desensitization.The Journal of physiology · 1999Article
- In vivo pathway of thermal hyperalgesia by intrathecal administration of alpha,beta-methylene ATP in mouse spinal cord: involvement of the glutamate-NMDA receptor system.British journal of pharmacology · 1999Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
1. ATP-gated non-selective cation channels from the rat vas deferens (P2X1 receptors) were stably expressed in HEK 293 cells, assayed by patch clamp on the first day after passage of the culture, and found to have whole-cell current kinetics markedly faster in both activation and desensitization than those found in the native vas deferens tissue, in agreement with previous reports. 2. By the second day after passage of the culture, however, the whole-cell current kinetics of the expressed receptors shifted, slowing in both activation and desensitization. The kinetic change correlated with a change in phenotype of the host cells from round to flat, and the slower kinetics were similar to native P2X1 currents recorded from dissociated rat vas deferens smooth muscle cells. Two point mutations in a pore-like domain near or within the second transmembrane domain of the P2X1 receptor appeared to confer on the receptor the inability to effect this change in kinetics over time. 3. Treatment of cells on day 3 after passage with cytochalasins B or D caused a reversion to the rapid kinetics phenotype, implicating the actin cytoskeleton in the development of the native kinetics. P2X1 receptors may therefore require interaction with an intact actin cytoskeleton for native kinetics, and the mutants may be defective either in interaction with the actin skeleton or in coupling the interaction to gating.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.