ArticlePlant physiology1998
The chloroplast atpA gene cluster in Chlamydomonas reinhardtii. Functional analysis of a polycistronic transcription unit.
Article in Plant physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 58 citations in OpenAlex.
- Engineering bidirectional chloroplast promoters for tunable co-expression of multiple genes in microalgae (Chlamydomonas reinhardtii).Communications biology · 2026Article
- Plastome diversity and phylogenetic insights among modern Egyptian wheat cultivars: Genome-Wide and Gene-Level perspectives.BMC plant biology · 2025Article
- Pas de Trois: An Overview of Penta-, Tetra-, and Octo-Tricopeptide Repeat Proteins FromFrontiers in plant science · 2021Review
- Intercistronic expression elements (IEE) from the chloroplast of Chlamydomonas reinhardtii can be used for the expression of foreign genes in synthetic operons.Plant molecular biology · 2018Article
- High-throughput sequencing of the chloroplast and mitochondrion of Chlamydomonas reinhardtii to generate improved de novo assemblies, analyze expression patterns and transcript speciation, and evaluate diversity among laboratory strains and wild isolates.The Plant journal : for cell and molecular biology · 2018Article
- Small RNA profiling in Chlamydomonas: insights into chloroplast RNA metabolism.Nucleic acids research · 2017Article
- Inferring the evolutionary mechanism of the chloroplast genome size by comparing whole-chloroplast genome sequences in seed plants.Scientific reports · 2017Article
- Spontaneous dominant mutations in chlamydomonas highlight ongoing evolution by gene diversification.The Plant cell · 2015Article
- Small RNAs reveal two target sites of the RNA-maturation factor Mbb1 in the chloroplast of Chlamydomonas.Nucleic acids research · 2014Article
- A new method to identify flanking sequence tags in chlamydomonas using 3'-RACE.Plant methods · 2012Article
- Manipulating RuBisCO accumulation in the green alga, Chlamydomonas reinhardtii.Plant molecular biology · 2011Article
- Molecular factors affecting the accumulation of recombinant proteins in the Chlamydomonas reinhardtii chloroplast.Molecular biotechnology · 2011Article
- MRL1, a conserved Pentatricopeptide repeat protein, is required for stabilization of rbcL mRNA in Chlamydomonas and Arabidopsis.The Plant cell · 2010Article
- Molecular identification and function of cis- and trans-acting determinants for petA transcript stability in Chlamydomonas reinhardtii chloroplasts.Molecular and cellular biology · 2008Article
- Integration of chloroplast nucleic acid metabolism into the phosphate deprivation response in Chlamydomonas reinhardtii.The Plant cell · 2007Article
- Microarray analysis confirms the specificity of a Chlamydomonas reinhardtii chloroplast RNA stability mutant.Plant physiology · 2005Article
- Biogenesis of PSI involves a cascade of translational autoregulation in the chloroplast of Chlamydomonas.The EMBO journal · 2004Article
- Chloroplast RNA processing and stability.Photosynthesis research · 2004Article
- Chlamydomonas reinhardtii at the crossroads of genomics.Eukaryotic cell · 2003Review
- The Chlamydomonas reinhardtii organellar genomes respond transcriptionally and post-transcriptionally to abiotic stimuli.The Plant cell · 2002Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most chloroplast genes in vascular plants are organized into polycistronic transcription units, which generate a complex pattern of mono-, di-, and polycistronic transcripts. In contrast, most Chlamydomonas reinhardtii chloroplast transcripts characterized to date have been monocistronic. This paper describes the atpA gene cluster in the C. reinhardtii chloroplast genome, which includes the atpA, psbI, cemA, and atpH genes, encoding the alpha-subunit of the coupling-factor-1 (CF1) ATP synthase, a small photosystem II polypeptide, a chloroplast envelope membrane protein, and subunit III of the CF0 ATP synthase, respectively. We show that promoters precede the atpA, psbI, and atpH genes, but not the cemA gene, and that cemA mRNA is present only as part of di-, tri-, or tetracistronic transcripts. Deletions introduced into the gene cluster reveal, first, that CF1-alpha can be translated from di- or polycistronic transcripts, and, second, that substantial reductions in mRNA quantity have minimal effects on protein synthesis rates. We suggest that posttranscriptional mRNA processing is common in C. reinhardtii chloroplasts, permitting the expression of multiple genes from a single promoter.
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