ArticlePlant physiology1998
Tissue culture-specific expression of a naturally occurring tobacco feedback-insensitive anthranilate synthase.
Article in Plant physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 53 citations in OpenAlex.
- OsASA2 Loss-of-Function Confers Broad-Spectrum Disease Resistance and Triggers Cell Death by Regulating Anthranilate Synthase Activity in Rice.Molecular plant pathology · 2026Article
- Anthranilate at the interface of tryptophan and specialized metabolite biosynthesis.Frontiers in plant science · 2025Review
- Evolution of aromatic amino acid metabolism in plants: a key driving force behind plant chemical diversity in aromatic natural products.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Deregulation of phenylalanine biosynthesis evolved with the emergence of vascular plants.Plant physiology · 2022Article
- Evolutionary Diversification of Primary Metabolism and Its Contribution to Plant Chemical Diversity.Frontiers in plant science · 2019Review
- Bean metal-responsive element-binding transcription factor confers cadmium resistance in tobacco.Plant physiology · 2015Article
- Emerging trends in research on spatial and temporal organization of terpenoid indole alkaloid pathway in Catharanthus roseus: a literature update.Protoplasma · 2012Review
- Tobacco plastid transformation using the feedback-insensitive anthranilate synthase [alpha]-subunit of tobacco (ASA2) as a new selectable marker.Journal of experimental botany · 2009Article
- Tissue culture specificity of the tobacco ASA2 promoter driving hpt as a selectable marker for soybean transformation selection.Plant cell reports · 2008Article
- Use of 4-methylindole or 7-methyl-DL-tryptophan in a transformant selection system based on the feedback-insensitive anthranilate synthase alpha-subunit of tobacco (ASA2).Plant cell reports · 2008Article
- Expression of a feedback insensitive anthranilate synthase gene from tobacco increases free tryptophan in soybean plants.Plant cell reports · 2007Article
- Article
- Structure-based in vitro engineering of the anthranilate synthase, a metabolic key enzyme in the plant tryptophan pathway.Plant physiology · 2005Article
- Characterization of the altered anthranilate synthase in 5-methyltryptophan-resistant rice mutants.Plant cell reports · 2005Article
- Molecular characterization of two anthranilate synthase alpha subunit genes in Camptotheca acuminata.Planta · 2005Article
- Overexpression of the feedback-insensitive anthranilate synthase gene in tobacco causes tryptophan accumulation.Plant cell reports · 2005Article
- Use of the tobacco feedback-insensitive anthranilate synthase gene (ASA2) as a selectable marker for legume hairy root transformation.Plant cell reports · 2004Article
- Targeting a nuclear anthranilate synthase alpha-subunit gene to the tobacco plastid genome results in enhanced tryptophan biosynthesis. Return of a gene to its pre-endosymbiotic origin.Plant physiology · 2001Article
- Characterization of rice anthranilate synthase alpha-subunit genes OASA1 and OASA2. Tryptophan accumulation in transgenic rice expressing a feedback-insensitive mutant of OASA1.Plant physiology · 2001Article
- Increasing tryptophan synthesis in a forage legume Astragalus sinicus by expressing the tobacco feedback-insensitive anthranilate synthase (ASA2) gene.Plant physiology · 2000Article
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Authors and funding
4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
A cDNA and corresponding promoter region for a naturally occurring, feedback-insensitive anthranilate synthase (AS) alpha-subunit gene, ASA2, has been isolated from an unselected, but 5-methyl-tryptophan-resistant (5MTr), tobacco (Nicotiana tabacum) cell line (AB15-12-1). The ASA2 cDNA contains a putative transit peptide sequence, and Southern hybridization shows that more than one closely related sequence is present in the tobacco genome. The ASA2 cDNA complemented a trpE nonsense mutant Escherichia coli strain, allowing growth on 300 microm 5MT-containing minimal medium without tryptophan, and cell extracts contained feedback-insensitive AS activity. The 5MTr was lost when the E. coli strain was transformed with an ASA2 site-directed mutant (phenylalanine-107-arginine-108 --> serine-107-glutamine-108). Identical nucleotide sequences encoding the phenylalanine-107-arginine-108 region have been found in polymerase chain reaction-amplified 326-bp ASA2 genomic fragments of wild-type (5-methyl-tryptophan-sensitive [5MTs]) tobacco and a progenitor species. High-level ASA2 transcriptional expression was detected only in 5MTr-cultured cells, not in 5MTs cells or in plants. Promoter studies indicate that tissue specificity of ASA2 is controlled by the promoter region between -2252 and -607. Since the ASA2 promoter sequences are not substantially different in the 5MTr and 5MTs lines, the increased levels of ASA2 mRNA in the 5MTr lines are most likely due to changes in a regulatory gene affecting ASA2 expression.
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