ArticlePlant physiology1998
Identification of the gene encoding the tryptophan synthase beta-subunit from Chlamydomonas reinhardtii.
Article in Plant physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- A cyclical marker system enables indefinite series of oligonucleotide-directed gene editing in Chlamydomonas reinhardtii.Plant physiology · 2024Article
- Applications of the MicroalgaeLife (Basel, Switzerland) · 2024Review
- Review
- miRNA-Mediated Regulation of Synthetic Gene Circuits in the Green Alga Chlamydomonas reinhardtii.ACS synthetic biology · 2019Article
- Azospirillum brasilense Increases COMicrobial ecology · 2018Article
- CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii.Scientific reports · 2016Article
- A NIMA-Related Kinase Suppresses the Flagellar Instability Associated with the Loss of Multiple Axonemal Structures.PLoS genetics · 2015Article
- Article
- A novel rhodanese is required to maintain chloroplast translation in Chlamydomonas.Plant molecular biology · 2012Article
- Article
- MicroCT-based virtual histology evaluation of preclinical medulloblastoma.Molecular imaging and biology · 2011Article
- Cucumber mosaic virus 2b protein inhibits RNA silencing pathways in green alga Chlamydomonas reinhardtii.Plant cell reports · 2010Article
- A ferroxidase encoded by FOX1 contributes to iron assimilation under conditions of poor iron nutrition in Chlamydomonas.Eukaryotic cell · 2008Article
- RNA silencing in Chlamydomonas: mechanisms and tools.Current genetics · 2006Review
- Mutant kinesin-2 motor subunits increase chromosome loss.Molecular biology of the cell · 2005Article
- Paths toward algal genomics.Plant physiology · 2005Review
- A genome's-eye view of the light-harvesting polypeptides of Chlamydomonas reinhardtii.Current genetics · 2004Review
- Chlamydomonas reinhardtii at the crossroads of genomics.Eukaryotic cell · 2003Review
- Article
- Molecular map of the Chlamydomonas reinhardtii nuclear genome.Eukaryotic cell · 2003Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
We report the isolation of a Chlamydomonas reinhardtii cDNA that encodes the beta-subunit of tryptophan synthase (TSB). This cDNA was cloned by functional complementation of a trp-operon-deleted strain of Escherichia coli. Hybridization analysis indicated that the gene exists in a single copy. The predicted amino acid sequence showed the greatest identity to TSB polypeptides from other photosynthetic organisms. With the goal of identifying mutations in the gene encoding this enzyme, we isolated 11 recessive and 1 dominant single-gene mutation that conferred resistance to 5-fluoroindole. These mutations fell into three complementation groups, MAA2, MAA7, and TAR1. In vitro assays showed that mutations at each of these loci affected TSB activity. Restriction fragment-length polymorphism analysis suggested that MAA7 encodes TSB. MAA2 and TAR1 may act to regulate the activity of MAA7 or its protein product.
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Registered trials
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