ArticleJournal of virology1998
Rabbit cells expressing human CD4 and human CCR5 are highly permissive for human immunodeficiency virus type 1 infection.
Article in Journal of virology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- The wide utility of rabbits as models of human diseases.Experimental & molecular medicine · 2018Review
- Rabbit Models for Studying Human Infectious Diseases.Comparative medicine · 2015Review
- Macaque-tropic human immunodeficiency virus type 1: breaking out of the host restriction factors.Frontiers in microbiology · 2013Article
- Rodent models for HIV-associated neurocognitive disorders.Trends in neurosciences · 2012Review
- Cytotoxic effect of HIV-1 gp120 on primary cultured human retinal capillary endothelial cells.Molecular vision · 2011Article
- High natural permissivity of primary rabbit cells for HIV-1, with a virion infectivity defect in macrophages as the final replication barrier.Journal of virology · 2010Article
- The antiretroviral potency of APOBEC1 deaminase from small animal species.Nucleic acids research · 2008Article
- An active TRIM5 protein in rabbits indicates a common antiviral ancestor for mammalian TRIM5 proteins.Journal of virology · 2007Article
- Primary T-cells from human CD4/CCR5-transgenic rats support all early steps of HIV-1 replication including integration, but display impaired viral gene expression.Retrovirology · 2007Article
- A phenotypic recessive, post-entry block in rabbit cells that results in aberrant trafficking of HIV-1.Traffic (Copenhagen, Denmark) · 2006Article
- Rodent cells support key functions of the human immunodeficiency virus type 1 pathogenicity factor Nef.Journal of virology · 2005Article
- Susceptibility of mink (Mustera vision)-derived cells to replication by human immunodeficiency virus type 1.Journal of virology · 2003Article
- Blockade of HIV-1 infection of New World monkey cells occurs primarily at the stage of virus entry.The Journal of experimental medicine · 2002Article
- Susceptibility of rat-derived cells to replication by human immunodeficiency virus type 1.Journal of virology · 2001Article
- Mouse-human heterokaryons support efficient human immunodeficiency virus type 1 assembly.Journal of virology · 2001Article
- A block to human immunodeficiency virus type 1 assembly in murine cells.Journal of virology · 2000Article
- V3 recombinants indicate a central role for CCR5 as a coreceptor in tissue infection by human immunodeficiency virus type 1.Journal of virology · 1999Article
- HIV type-1 infection of the cotton rat (Sigmodon fulviventer and S. hispidus).Proceedings of the National Academy of Sciences of the United States of America · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
To evaluate the feasibility of using transgenic rabbits expressing CCR5 and CD4 as a small-animal model of human immunodeficiency virus type 1 (HIV) disease, we examined whether the expression of the human chemokine receptor (CCR5) and human CD4 would render a rabbit cell line (SIRC) permissive to HIV replication. Histologically, SIRC cells expressing CD4 and CCR5 formed multinucleated cells (syncytia) upon exposure to BaL, a macrophagetropic strain of HIV that uses CCR5 for cell entry. Intracellular viral capsid p24 staining showed abundant viral gene expression in BaL-infected SIRC cells expressing CD4 and CCR5. In contrast, neither SIRC cells expressing CD4 alone nor murine 3T3 cells expressing CCR5 and CD4 exhibited significant expression of p24. These stably transfected rabbit cells were also highly permissive for the production of virions upon infection by two other CCR5-dependent strains (JR-CSF and YU-2) but not by a CXCR4-dependent strain (NL4-3). The functional integrity of these virions was demonstrated by the successful infection of human peripheral blood mononuclear cells (PBMC) with viral stocks prepared from these transfected rabbit cells. Furthermore, primary rabbit PBMC were found to be permissive for production of infectious virions after circumventing the cellular entry step. These results suggest that a transgenic rabbit model for the study of HIV disease may be feasible.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.