ArticleJournal of bacteriology1998
Multidomain structure and cellulosomal localization of the Clostridium thermocellum cellobiohydrolase CbhA.
Article in Journal of bacteriology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 73 citations in OpenAlex.
- Novel Insights from Comparative In Silico Analysis of Green Microalgal Cellulases.International journal of molecular sciences · 2018Article
- Handling gene and protein names in the age of bioinformatics: the special challenge of secreted multimodular bacterial enzymes such as the cbhA/cbh9A gene of Clostridium thermocellum.World journal of microbiology & biotechnology · 2018Review
- Optimizing the composition of a synthetic cellulosome complex for the hydrolysis of softwood pulp: identification of the enzymatic core functions and biochemical complex characterization.Biotechnology for biofuels · 2018Article
- Comparative characterization of all cellulosomal cellulases fromBiotechnology for biofuels · 2017Article
- Enzymatic diversity of the Clostridium thermocellum cellulosome is crucial for the degradation of crystalline cellulose and plant biomass.Scientific reports · 2016Article
- Multiple cellobiohydrolases and cellobiose phosphorylases cooperate in the ruminal bacterium Ruminococcus albus 8 to degrade cellooligosaccharides.Scientific reports · 2016Article
- Glycosylated linkers in multimodular lignocellulose-degrading enzymes dynamically bind to cellulose.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Modeling the self-assembly of the cellulosome enzyme complex.The Journal of biological chemistry · 2011Article
- Global view of the Clostridium thermocellum cellulosome revealed by quantitative proteomic analysis.Journal of bacteriology · 2007Article
- Complete cellulase system in the marine bacterium Saccharophagus degradans strain 2-40T.Journal of bacteriology · 2006Article
- Cellulase, clostridia, and ethanol.Microbiology and molecular biology reviews : MMBR · 2005Review
- Hydrophilic domains of scaffolding protein CbpA promote glycosyl hydrolase activity and localization of cellulosomes to the cell surface of Clostridium cellulovorans.Journal of bacteriology · 2004Article
- Ruminococcus albus 8 mutants defective in cellulose degradation are deficient in two processive endocellulases, Cel48A and Cel9B, both of which possess a novel modular architecture.Journal of bacteriology · 2004Article
- Calcium and domain interactions contribute to the thermostability of domains of the multimodular cellobiohydrolase, CbhA, a subunit of the Clostridium thermocellum cellulosome.The Biochemical journal · 2003Article
- Microbial cellulose utilization: fundamentals and biotechnology.Microbiology and molecular biology reviews : MMBR · 2002Review
- The fibronectin type 3-like repeat from the Clostridium thermocellum cellobiohydrolase CbhA promotes hydrolysis of cellulose by modifying its surface.Applied and environmental microbiology · 2002Article
- EndB, a multidomain family 44 cellulase from Ruminococcus flavefaciens 17, binds to cellulose via a novel cellulose-binding module and to another R. flavefaciens protein via a dockerin domain.Applied and environmental microbiology · 2001Article
- Properties and mutation analysis of the CelK cellulose-binding domain from the Clostridium thermocellum cellulosome.Journal of bacteriology · 2001Article
- Advances in development of a genetic system for Thermoanaerobacterium spp.: expression of genes encoding hydrolytic enzymes, development of a second shuttle vector, and integration of genes into the chromosome.Applied and environmental microbiology · 2000Article
- CelE, a multidomain cellulase from Clostridium cellulolyticum: a key enzyme in the cellulosome?Journal of bacteriology · 2000Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nucleotide sequence of the Clostridium thermocellum F7 cbhA gene, coding for the cellobiohydrolase CbhA, has been determined. An open reading frame encoding a protein of 1,230 amino acids was identified. Removal of a putative signal peptide yields a mature protein of 1,203 amino acids with a molecular weight of 135,139. Sequence analysis of CbhA reveals a multidomain structure of unusual complexity consisting of an N-terminal cellulose binding domain (CBD) homologous to CBD family IV, an immunoglobulin-like beta-barrel domain, a catalytic domain homologous to cellulase family E1, a duplicated domain similar to fibronectin type III (Fn3) modules, a CBD homologous to family III, a highly acidic linker region, and a C-terminal dockerin domain. The cellulosomal localization of CbhA was confirmed by Western blot analysis employing polyclonal antibodies raised against a truncated enzymatically active version of CbhA. CbhA was identified as cellulosomal subunit S3 by partial amino acid sequence analysis. Comparison of the multidomain structures indicates striking similarities between CbhA and a group of cellulases from actinomycetes. Average linkage cluster analysis suggests a coevolution of the N-terminal CBD and the catalytic domain and its spread by horizontal gene transfer among gram-positive cellulolytic bacteria.
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