ArticleJournal of clinical microbiology1998
Rapid extraction of genomic DNA from medically important yeasts and filamentous fungi by high-speed cell disruption.
Article in Journal of clinical microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
59 citing papers in PubMed, 154 citations in OpenAlex.
- Exploring the Genetic Diversity of Candida albicans in Animal Hosts: A One Health Perspective.Zoonoses and public health · 2026Article
- Structure of ergosteryl-aspartate synthase reveals how an entrapped tRNA is used like a prosthetic swinging arm in the synthesis of aminoacylated sterols.Nature communications · 2026Article
- Novel Freshwater Ascomycetes from Submerged Plant Debris in the Zújar River (Extremadura Community, Spain).Journal of fungi (Basel, Switzerland) · 2026Article
- Occurrence of toxigenic fungi and mycotoxins in pig feeds: potential implications for workers' exposure.Mycotoxin research · 2026Article
- Genomic epidemiology and phylogenomics ofFrontiers in cellular and infection microbiology · 2026Article
- Article
- Molecular characterization and virulence of fungal pathogens associated with mass mortalities in hilsa Shad (Tenualosa ilisha).Scientific reports · 2025Article
- New Species of Ascomycetes from Two Hypersaline Endorheic Lagoon Complexes in Zaragoza Province (Aragon Community, Spain).Journal of fungi (Basel, Switzerland) · 2025Article
- Trends in Prevalence of Yeast Species Associated With Urogenital Infection in Nsukka, Nigeria: An Overview of TrueInternational journal of microbiology · 2025Article
- Sunken Riches: Ascomycete Diversity in the Western Mediterranean Coast through Direct Plating and Flocculation, and Description of Four New Taxa.Journal of fungi (Basel, Switzerland) · 2024Article
- A New Genus of theJournal of fungi (Basel, Switzerland) · 2024Article
- Insights into Some Onygenalean Fungi from Freshwater Sediments in Spain and Description of Novel Taxa.Journal of fungi (Basel, Switzerland) · 2023Article
- Diversity and novel lineages of black yeasts inPersoonia · 2023Article
- Review
- Automation protocol for high-efficiency and high-quality genomic DNA extraction from Saccharomyces cerevisiae.PloS one · 2023Article
- Genotyping ofCurrent medical mycology · 2022Article
- Article
- Two Novel Genera,Journal of fungi (Basel, Switzerland) · 2022Article
- High molecular weight DNA extraction methods lead to high quality filamentous ascomycete fungal genome assemblies using Oxford Nanopore sequencing.Microbial genomics · 2022Article
- Hydrolytic Enzymes as Potentiators of Antimicrobials against an Inter-Kingdom Biofilm Model.Microbiology spectrum · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Current methods of DNA extraction from different fungal pathogens are often time-consuming and require the use of toxic chemicals. DNA isolation from some fungal organisms is difficult due to cell walls or capsules that are not readily susceptible to lysis. We therefore investigated a new and rapid DNA isolation method using high-speed cell disruption (HSCD) incorporating chaotropic reagents and lysing matrices in comparison to standard phenol-chloroform (PC) extraction protocols for isolation of DNA from three medically important yeasts (Candida albicans, Cryptococcus neoformans, and Trichosporon beigelii) and two filamentous fungi (Aspergillus fumigatus and Fusarium solani). Additional extractions by HSCD were performed on Saccharomyces cerevisiae, Pseudallescheria boydii, and Rhizopus arrhizus. Two different inocula (10(8) and 10(7) CFU) were compared for optimization of obtained yields. The entire extraction procedure was performed on as many as 12 samples within 1 h compared to 6 h for PC extraction. In comparison to the PC procedure, HSCD DNA extraction demonstrated significantly greater yields for 10(8) CFU of C. albicans, T. beigelii, A. fumigatus, and F. solani (P < or = 0.005), 10(7) CFU of C. neoformans (P < or = 0.05), and 10(7) CFU of A. fumigatus (P < or = 0.01). Yields were within the same range for 10(8) CFU of C. neoformans and l0(7) CFU of C. albicans for both HSCD extraction and PC extraction. For 10(7) CFU of T. beigelii, PC extraction resulted in a greater yield than did HSCD (P < or = 0.05). Yields obtained from 10(8) and 10(7) CFU were significantly greater for filamentous fungi than for yeasts by the HSCD extraction procedure (P < 0.0001). By the PC extraction procedure, differences were not significant. For all eight organisms, the rapid extraction procedure resulted in good yield, integrity, and quality of DNA as demonstrated by restriction fragment length polymorphism, PCR, and random amplified polymorphic DNA. We conclude that mechanical disruption of fungal cells by HSCD is a safe, rapid, and efficient procedure for extracting genomic DNA from medically important yeasts and especially from filamentous fungi.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.