ArticleJournal of clinical microbiology1998
Identification of new influenza B virus variants by multiplex reverse transcription-PCR and the heteroduplex mobility assay.
Article in Journal of clinical microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
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- Pathogenicity and transmissibility assessment of two strains of human influenza virus isolated in China in 2018.The Journal of international medical research · 2021Article
- Quantification of designer nuclease induced mutation rates: a direct comparison of different methods.Molecular therapy. Methods & clinical development · 2016Article
- An efficient genotyping method for genome-modified animals and human cells generated with CRISPR/Cas9 system.Scientific reports · 2014Article
- Chemically modified primers for improved multiplex polymerase chain reaction.Analytical biochemistry · 2009Article
- Rapid differentiation of influenza A virus subtypes and genetic screening for virus variants by high-resolution melting analysis.Journal of clinical microbiology · 2008Article
- Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products.Nucleic acids research · 2005Article
- Sensitive detection of SARS coronavirus RNA by a novel asymmetric multiplex nested RT-PCR amplification coupled with oligonucleotide microarray hybridization.Methods in molecular medicine · 2005Article
- Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus.Laboratory investigation; a journal of technical methods and pathology · 2004Article
- Sequencing-based detection of low-frequency human immunodeficiency virus type 1 drug-resistant mutants by an RNA/DNA heteroduplex generator-tracking assay.Journal of virology · 2004Article
- Influenza AH1N2 viruses, United Kingdom, 2001-02 influenza season.Emerging infectious diseases · 2003Article
- Multiplex PCR: rapid DNA cycling in a conventional thermal cycler.Journal of clinical laboratory analysis · 2003Article
- Combined PCR-heteroduplex mobility assay for detection and differentiation of influenza A viruses from different animal species.Journal of clinical microbiology · 2001Article
- Use of a heteroduplex mobility assay to detect differences in the fusion protein cleavage site coding sequence among Newcastle disease virus isolates.Journal of clinical microbiology · 2001Article
- Multiplex PCR: optimization and application in diagnostic virology.Clinical microbiology reviews · 2000Review
- Simplified hepatitis C virus genotyping by heteroduplex mobility analysis.Journal of clinical microbiology · 2000Article
- Use of base excision sequence scanning for detection of genetic variations in St. Louis encephalitis virus isolates.Journal of clinical microbiology · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A quick genetic approach for the screening of influenza virus variants was developed in this laboratory (S. Zou, J. Clin. Microbiol. 35:2623-2627, 1997). It uses multiplex reverse transcription and multiplex PCR to amplify and differentiate the variable region of the hemagglutinin genes of different types and subtypes of influenza viruses. Variants within the same type or subtype are then identified by the heteroduplex mobility shift assay of the amplicons. The method was used to screen influenza virus isolates received from provincial laboratories during the 1996-1997 season and was able to identify new influenza B virus variants. Sequencing of the amplicons derived from the hemagglutinin gene of the identified variants and comparison with the vaccine strain B/Harbin/7/94 showed substitution rates of 2.26 to 2.55% at the nucleotide level and 4.26 to 4.68% at the amino acid level. The result further demonstrated that the approach provides a quick, sensitive, and reliable screening for influenza virus variants. It also suggested the necessity of close monitoring of influenza B virus isolates in the 1997-1998 season and critical evaluation of the reference strain for the type B influenza virus.
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