ArticlePlant molecular biology1998
Characterization of a single soybean cDNA encoding cytosolic and glyoxysomal isozymes of aspartate aminotransferase.
Article in Plant molecular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 24 citations in OpenAlex.
- Network analysis combined with genome-wide association study helps identification of genes related to amino acid contents in soybean.BMC genomics · 2025Article
- The functional readthrough extension of malate dehydrogenase reveals a modification of the genetic code.Open biology · 2016Article
- Proteome analysis of peroxisomes from etiolated Arabidopsis seedlings identifies a peroxisomal protease involved in β-oxidation and development.Plant physiology · 2013Article
- Identification of QTL in soybean underlying resistance to herbivory by Japanese beetles (Popillia japonica, Newman).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2010Article
- Arabidopsis glutathione reductase 1 is dually targeted to peroxisomes and the cytosol.Plant signaling & behavior · 2010Article
- Matching the proteome to the genome: the microbody of penicillin-producing Penicillium chrysogenum cells.Functional & integrative genomics · 2009Article
- Metabolite transport across the peroxisomal membrane.The Biochemical journal · 2007Review
- Development of a pooled probe method for locating small gene families in a physical map of soybean using stress related paralogues and a BAC minimum tile path.Plant methods · 2006Article
- AraPerox. A database of putative Arabidopsis proteins from plant peroxisomes.Plant physiology · 2004Article
- Import of the peroxisomal targeting signal type 2 protein 3-ketoacyl-coenzyme a thiolase into glyoxysomes.Plant physiology · 2003Article
- The putative glutamate receptor 1.1 (AtGLR1.1) functions as a regulator of carbon and nitrogen metabolism in Arabidopsis thaliana.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A soybean cDNA clone, pSAT1, which encodes both the cytosolic and glyoxysomal isozymes of aspartate aminotransferase (AAT; EC 2.6.1.1) was isolated. Genomic Southern blots and analysis of genomic clones indicated pSAT1 was encoded by a single copy gene. pSAT1 contained an open reading frame with ca. 90% amino acid identity to alfalfa and lupin cytosolic AAT and two in-frame start codons, designated ATG1 and ATG2. Alignment of this protein with other plant cytosolic AAT isozymes revealed a 37 amino acid N-terminal extension with characteristics of a peroxisomal targeting signal, designated PTS2, including the modified consensus sequence RL-X5-HF. The second start codon ATG2 aligned with previously reported start codons for plant cytosolic AAT cDNAs. Plasmids constructed to express the open reading frame initiated by each of the putative start codons produced proteins with AAT activity in Escherichia coli. Immune serum raised against the pSAT1-encoded protein reacted with three soybean AAT isozymes, AAT1 (glyoxysomal), AAT2 (cytosolic), and AAT3 (subcellular location unknown). We propose the glyoxysomal isozyme AAT1 is produced by translational initiation from ATG1 and the cytosolic isozyme AAT2 is produced by translational initiation from ATG2. N-terminal sequencing of purified AAT1 revealed complete identity with the pSAT1-encoded protein and was consistent with the processing of the PTS2. Analysis of cytosolic AAT genomic sequences from several other plant species revealed conservation of the two in-frame start codons and the PTS2 sequence, suggesting that these other species may utilize a single gene to generate both cytosolic and glyoxysomal or peroxisomal forms of AAT.
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