ArticleProceedings of the National Academy of Sciences of the United States of America1998
Transgene organization in rice engineered through direct DNA transfer supports a two-phase integration mechanism mediated by the establishment of integration hot spots.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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56 citing papers in PubMed, 262 citations in OpenAlex.
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- Expression dynamics of mCry3A and eCry3.1Ab transgenes in Bt corn hybrids across growth and environments.Transgenic research · 2026Article
- Toward the development of Ac/Ds transposon-mediated gene tagging system for functional genomics in oat (Avena sativa L.).Functional & integrative genomics · 2022Article
- Studies on Pure MlbFrontiers in plant science · 2022Article
- Silver nanoparticles for biolistic transformation in3 Biotech · 2021Article
- Particle bombardment technology and its applications in plants.Molecular biology reports · 2020Review
- Low-temperature pretreatment of explants and high maltose concentration during callus culture improves particle-bombardment-mediated stable transgene expression in common wheat.Plant biotechnology (Tokyo, Japan) · 2020Article
- Genomic variation between PRSV resistant transgenic SunUp and its progenitor cultivar Sunset.BMC genomics · 2020Article
- Nanobiolistics: An Emerging Genetic Transformation Approach.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Metabolic Engineering Strategies in Diatoms Reveal Unique Phenotypes and Genetic Configurations With Implications for Algal Genetics and Synthetic Biology.Frontiers in bioengineering and biotechnology · 2020Article
- Coexpression of octopine and succinamopine Agrobacterium virulence genes to generate high quality transgenic events in maize by reducing vector backbone integration.Transgenic research · 2018Article
- MAR-Mediated transgene integration into permissive chromatin and increased expression by recombination pathway engineering.Biotechnology and bioengineering · 2017Article
- Developing transgenic wheat to encounter rusts and powdery mildew by overexpressing barleyPlant methods · 2017Article
- The distribution of cotransformed transgenes in particle bombardment-mediated transformed wheat.Transgenic research · 2015Article
- Strategies for metabolic pathway engineering with multiple transgenes.Plant molecular biology · 2013Review
- HvCKX2 gene silencing by biolistic or Agrobacterium-mediated transformation in barley leads to different phenotypes.BMC plant biology · 2012Article
- Genetic approaches for studying transgene inheritance and genetic recombination in three successive generations of transformed tobacco.Genetics and molecular biology · 2012Article
- Phospholipid and triacylglycerol profiles modified by PLD suppression in soybean seed.Plant biotechnology journal · 2011Article
- Resistance to Cucumber mosaic virus in Gladiolus plants transformed with either a defective replicase or coat protein subgroup II gene from Cucumber mosaic virus.Plant cell reports · 2010Article
- Dosage-dependent gene expression from direct repeat locus in rice developed by site-specific gene integration.Molecular biotechnology · 2010Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Organization of transgenes in rice transformed through direct DNA transfer strongly suggests a two-phase integration mechanism. In the "preintegration" phase, transforming plasmid molecules (either intact or partial) are spliced together. This gives rise to rearranged transgenic sequences, which upon integration do not contain any interspersed plant genomic sequences. Subsequently, integration of transgenic DNA into the host genome is initiated. Our experiments suggest that the original site of integration acts as a hot spot, facilitating subsequent integration of successive transgenic molecules at the same locus. The resulting transgenic locus may have plant DNA separating the transgenic sequences. Our data indicate that transformation through direct DNA transfer, specifically particle bombardment, generally results in a single transgenic locus as a result of this two-phase integration mechanism. Transgenic plants generated through such processes may, therefore, be more amenable to breeding programs as the single transgenic locus will be easier to characterize genetically. Results from direct DNA transfer experiments suggest that in the absence of protein factors involved in exogenous DNA transfer through Agrobacterium, the qualitative and/or quantitative efficiency of transformation events is not compromised. Our results cast doubt on the role of Agrobacterium vir genes in the integration process.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.