ArticleProceedings of the National Academy of Sciences of the United States of America1998
Terpenoid-based defenses in conifers: cDNA cloning, characterization, and functional expression of wound-inducible (E)-alpha-bisabolene synthase from grand fir (Abies grandis).
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 185 citations in OpenAlex.
- Insight into TPS-d Subfamily: The Evolutionary and Functional Diversity in Gymnosperms.Biomolecules · 2026Review
- Chromosome-scale genome assembly of Artabotrys hexapetalus provides insights into its evolution and terpenoid biosynthesis.BMC plant biology · 2026Article
- Terpene-based biofuels production: a revolutionizing role of cyanobacteria through metabolic engineering, opportunities and challenges.Archives of microbiology · 2026Review
- Identification of a volatile chemotype associated with resilience to water stress in domesticated varieties of cotton.BMC biology · 2026Article
- Isolation and Characterization of Two Monoterpene Synthases and a Sesquiterpene Synthase fromMetabolites · 2025Article
- Production of the terpenoid-based biofuel α-bisabolene by metabolically engineered Corynebacterium glutamicum.World journal of microbiology & biotechnology · 2025Article
- Engineering Yeast Peroxisomes for α-Bisabolene Production from Sole Methanol with the Aid of Proteomic Analysis.JACS Au · 2024Article
- In Silico Genome-Wide Mining and Analysis of Terpene Synthase Gene Family in Hevea Brasiliensis.Biochemical genetics · 2023Article
- Genome mining of cryptic bisabolenes that were biosynthesized by intramembrane terpene synthases fromPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2023Article
- Over-expression of α-bisabolene by metabolic engineering of Yarrowia lipolytica employing a golden gate DNA assembly toolbox.Biotechnology notes (Amsterdam, Netherlands) · 2023Article
- Functional mining of novel terpene synthases from metagenomes.Biotechnology for biofuels and bioproducts · 2022Article
- Farnesyl pyrophosphate compartmentalization in the green microalga Chlamydomonas reinhardtii during heterologous (E)-α-bisabolene production.Microbial cell factories · 2022Article
- Insights into the biotechnology potential ofFrontiers in microbiology · 2022Review
- Evolution of Terpene Synthases in Orchidaceae.International journal of molecular sciences · 2021Review
- Metabolic engineering ofMetabolic engineering communications · 2021Article
- Article
- A molecular and genomic reference system for conifer defence against insects.Plant, cell & environment · 2019Review
- Terpene profiling, transcriptome analysis and characterization ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Review
- Intron-containing algal transgenes mediate efficient recombinant gene expression in the green microalga Chlamydomonas reinhardtii.Nucleic acids research · 2018Article
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
(E)-alpha-Bisabolene synthase is one of two wound-inducible sesquiterpene synthases of grand fir (Abies grandis), and the olefin product of this cyclization reaction is considered to be the precursor in Abies species of todomatuic acid, juvabione, and related insect juvenile hormone mimics. A cDNA encoding (E)-alpha-bisabolene synthase was isolated from a wound-induced grand fir stem library by a PCR-based strategy and was functionally expressed in Escherichia coli and shown to produce (E)-alpha-bisabolene as the sole product from farnesyl diphosphate. The expressed synthase has a deduced size of 93.8 kDa and a pI of 5. 03, exhibits other properties typical of sesquiterpene synthases, and resembles in sequence other terpenoid synthases with the exception of a large amino-terminal insertion corresponding to Pro81-Val296. Biosynthetically prepared (E)-alpha-[3H]bisabolene was converted to todomatuic acid in induced grand fir cells, and the time course of appearance of bisabolene synthase mRNA was shown by Northern hybridization to lag behind that of mRNAs responsible for production of induced oleoresin monoterpenes. These results suggest that induced (E)-alpha-bisabolene biosynthesis constitutes part of a defense response targeted to insect herbivores, and possibly fungal pathogens, that is distinct from induced oleoresin monoterpene production.
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