ArticlePlant molecular biology1998
The molecular basis of C4 photosynthesis in sorghum: isolation, characterization and RFLP mapping of mesophyll- and bundle-sheath-specific cDNAs obtained by differential screening.
Article in Plant molecular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 47 citations in OpenAlex.
- Proximity Labeling in Plants.Annual review of plant biology · 2023Review
- Most photorespiratory genes are preferentially expressed in the bundle sheath cells of the C4 grass Sorghum bicolor.Journal of experimental botany · 2016Article
- Getting the most out of natural variation in C4 photosynthesis.Photosynthesis research · 2014Review
- Article
- Comparative genomic analysis of C4 photosynthetic pathway evolution in grasses.Genome biology · 2009Article
- The activities of PEP carboxylase and the C4 acid decarboxylases are little changed by drought stress in three C4 grasses of different subtypes.Photosynthesis research · 2008Article
- A multi-treatment experimental system to examine photosynthetic differentiation in the maize leaf.BMC genomics · 2007Article
- Identification of C4 responsive genes in the facultative C4 plant Hydrilla verticillata.Photosynthesis research · 2006Article
- Nature's green revolution: the remarkable evolutionary rise of C4 plants.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2006Review
- Functional differentiation of bundle sheath and mesophyll maize chloroplasts determined by comparative proteomics.The Plant cell · 2005Article
- Use of Scots pine seedling roots as an experimental model to investigate gene expression during interaction with the conifer pathogen Heterobasidion annosum (P-type).Journal of plant research · 2004Article
- The evolution of CThe New phytologist · 2004Review
- Evolution of c4 phosphoenolpyruvate carboxylase. Genes and proteins: a case study with the genus Flaveria.Annals of botany · 2004Review
- Article
- Structure and properties of an engineered transketolase from maize.Plant physiology · 2003Article
- Toward integration of comparative genetic, physical, diversity, and cytomolecular maps for grasses and grains, using the sorghum genome as a foundation.Plant physiology · 2001Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
C4 photosynthesis depends upon the strict compartmentalization of the CO2-assimilatory enzymes of the C4 and Calvin cycle in two different cell types, mesophyll and bundle-sheath cells. A differential accumulation is also observed for enzymes of other metabolic pathways, and mesophyll and bundle-sheath chloroplasts of NADP-malic enzyme type C4 plants differ even in their photosynthetic electron transport chains. A large number of studies indicate that this division of labour between mesophyll and bundle-sheath cells is the result of differential gene expression. To investigate the extent of this differential gene expression and thus gain insight into the genetic basis of C4 photosynthesis, genes that are differentially expressed in the mesophyll and bundle-sheath cells were catalogued in the NADP-malic enzyme type C4 grass Sorghum bicolor. A total of 58 cDNAs were isolated by differential screening. Using a tenfold difference in transcript abundance between mesophyll and bundle-sheath cells as a criterion, 25 cDNAs were confirmed to encode mesophyll-specific gene sequences and 8 were found to encode bundle-sheath-specific sequences. Eight mesophyll-specific cDNAs showed no significant similarities within GenBank and may therefore represent candidates for the elucidation of hitherto unknown functions in the differentiation of mesophyll and bundle-sheath cells. The chromosomal location of 50 isolated cDNAs was determined by RFLP mapping using an interspecific sorghum cross.
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