ArticlePlant molecular biology1998
Structure, expression and localization of a germin-like protein in barley (Hordeum vulgare L.) that is insolubilized in stressed leaves.
Article in Plant molecular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 87 citations in OpenAlex.
- Molecular Characterization of Germin-like Protein Genes inACS omega · 2023Article
- Potential stress tolerance roles of barley germins and GLPs.Development genes and evolution · 2021Article
- Article
- Molecular evidence for the involvement of cotton GhGLP2, in enhanced resistance to Verticillium and Fusarium Wilts and oxidative stress.Scientific reports · 2020Article
- GhABP19, a Novel Germin-Like Protein FromFrontiers in plant science · 2019Article
- Long-term cadmium exposure influences the abundance of proteins that impact the cell wall structure in Medicago sativa stems.Plant biology (Stuttgart, Germany) · 2018Article
- Overexpression of Heat Shock Factor Gene HsfA3 Increases Galactinol Levels and Oxidative Stress Tolerance in Arabidopsis.Molecules and cells · 2016Article
- Maize-Pathogen Interactions: An Ongoing Combat from a Proteomics Perspective.International journal of molecular sciences · 2015Review
- Versatility of germin-like proteins in their sequences, expressions, and functions.Functional & integrative genomics · 2015Article
- Evolutionary relationships among barley and Arabidopsis core circadian clock and clock-associated genes.Journal of molecular evolution · 2015Article
- Do Cupins Have a Function Beyond Being Seed Storage Proteins?Frontiers in plant science · 2015Article
- Characterization of peanut germin-like proteins, AhGLPs in plant development and defense.PloS one · 2013Article
- Article
- Identification and analysis of the germin-like gene family in soybean.BMC genomics · 2010Article
- Regulation of two germin-like protein genes during plum fruit development.Journal of experimental botany · 2010Article
- Regulation of gene expression by chromosome 5A during cold hardening in wheat.Molecular genetics and genomics : MGG · 2010Article
- Physiological and molecular changes in Oryza meridionalis Ng., a heat-tolerant species of wild rice.Journal of experimental botany · 2010Article
- Tissue-specific expression of a defence-related peroxidase in transgenic wheat potentiates cell death in pathogen-attacked leaf epidermis.Molecular plant pathology · 2008Article
- The multigene family encoding germin-like proteins of barley. Regulation and function in Basal host resistance.Plant physiology · 2006Article
- Germin-like genes are expressed during somatic embryogenesis and early development of conifers.Plant molecular biology · 2006Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The primary leaves of young barley seedlings contain two major, extracellular, acid-soluble proteins of ca. 22 and 23 kDa apparent molecular mass. These proteins disappeared from the intercellular washing fluid upon stress treatments that enhanced H2O2 levels and that induced resistance to subsequent challenge by the powdery mildew fungus Erysiphe graminis f. sp. hordei. A partial peptide sequence of the 22 kDa protein was determined, and a cDNA clone was isolated. The 22 kDa protein belongs the the group of germin-like proteins (GLPs) and was designated HvGLP1. Despite its similarity to germin, i.e. oxalate oxidase, no oxalate oxidase activity of HvGLP1 could be detected. The RNA and soluble protein of HvGLP1 was highly abundant in young leaves, less abundant in older leaves and absent in roots. HvGLP1 RNA oscillated with a circadian rhythm, the minimum and maximum of RNA abundance being at the end of the dark and light periods, respectively. Heat and H2O2 treatment as well as pathogen infection caused disappearance of HvGLP1 protein from the fraction of soluble proteins of the intercellular space. HvGLP1 protein could be re-solubilized from cell walls of heat- or H2O2-treated leaves by boiling in SDS suggesting non-covalent cross linking. Although a physiological role of HvGLP1 insolubilization is still open, the protein may serve as marker for oxidative stress in cereals.
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