ArticleImmunology1998
Comparative cytokine gene expression: regulation and release by human mast cells.
Article in Immunology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 121 citations in OpenAlex.
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- Mast Cells and Angiogenesis in Human Plasma Cell Malignancies.International journal of molecular sciences · 2019Review
- Allergen-specific IgG antibody signaling through FcγRIIb promotes food tolerance.The Journal of allergy and clinical immunology · 2018Article
- Xanthii Fructus inhibits allergic response in the ovalbumin-sensitized mouse allergic rhinitis model.Pharmacognosy magazine · 2015Article
- Forsythia fructus inhibits the mast-cell-mediated allergic inflammatory reactions.Inflammation · 2003Article
- Manipulation of cell surface macromolecules by flaviviruses.Advances in virus research · 2003Review
- Correlation of bone marrow angiogenesis and mast cells with tryptase activity in myelodysplastic syndromes.Leukemia · 2002Article
- Dengue virus selectively induces human mast cell chemokine production.Journal of virology · 2002Article
- Cytokines regulate development of human mast cells from hematopoietic progenitors.International journal of hematology · 2002Review
- Inhibition by glucocorticoids of the interleukin-1beta-enhanced expression of the mast cell growth factor SCF.British journal of pharmacology · 2002Article
- Chemokines in the limbal form of vernal keratoconjunctivitis.The British journal of ophthalmology · 2000Article
- MEKK2 gene disruption causes loss of cytokine production in response to IgE and c-Kit ligand stimulation of ES cell-derived mast cells.The EMBO journal · 2000Article
- Release of vasoactive cytokines by antibody-enhanced dengue virus infection of a human mast cell/basophil line.Journal of virology · 2000Article
- The role of the vascular phase in solid tumor growth: a historical review.Neoplasia (New York, N.Y.) · 1999Article
- Adhesion of human mast cells to extracellular matrix provides a co-stimulatory signal for cytokine production.Immunology · 1999Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since data on the ability of human mast cells to produce various cytokines are scanty, we examined the mRNA expression, its modulation and the resulting protein expression of a number of well-characterized cytokines, using semi-quantitative reverse transcription-polymerase chain reaction of cell extracts and enzyme-linked immunosorbent assays for analysis of cell supernatants. One million cells/ml of the human mast cell line HMC-1 were stimulated with 25 ng/ml phorbol myristate acetate (PMA), 5 x 10(-7) M calcium ionophore A 23187 (ionophore) or both stimuli combined for various time periods. Constitutive expression in unstimulated cells was found for interleukin-1 beta (IL-1 beta) -3, -4, -8, tumour necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta (TGF-beta). Maximal mRNA up-regulation was observed by 2-4 hr, with a second peak for TNF-alpha at 24 hr. After a 4-hr stimulation, IL-13 expression was detectable as well, whereas for IL-12, only the p35 but not the p40 chain was found, and IL-2, -5, -7 and interferon-gamma (IFN-gamma) were not expressed at all. Large quantities of IL-8, TNF-alpha, granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-3 were secreted time-dependently over a 72-hr period, with lower levels of IL-1 beta, -6, -10 and TGF-beta and no detectable IL-2, -4 and IFN-gamma protein. When IL-6 and IL-8 expression was compared in more detail, IL-6 mRNA was found to be up-regulated only with ionophore but not PMA, whereas both stimuli alone or combined increased IL-8 mRNA expression. Preincubation with cycloheximide inhibited IL-6 but not IL-8 transcription, and incubation of stimulated cells with actinomycin D stabilized IL-8 and also IL-6 mRNA. These data suggest a selective regulation of distinct cytokines in human mast cells at the transcriptional and post-transcriptional levels. Furthermore, the spectrum of cytokines produced by HMC-1 cells supports the well-recognized role of mast cells in immediate-type hypersensitivity reactions as well as their potential colony-stimulating and tissue-remodelling abilities.
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