ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1998
Inhibition of CPP32-like proteases rescues axotomized retinal ganglion cells from secondary cell death in vivo.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
52 citing papers in PubMed, 194 citations in OpenAlex.
- Caspase-mediated pathways in retinal ganglion cell injury: a novel therapeutic target for glaucoma.Frontiers in cell and developmental biology · 2025Review
- Ouabain-NaNeurochemical research · 2022Article
- Review
- Fasudil attenuates glial cell-mediated neuroinflammation via ERK1/2 and AKT signaling pathways after optic nerve crush.Molecular biology reports · 2020Article
- Pharmacological Effects of Melatonin as Neuroprotectant in Rodent Model: A Review on the Current Biological Evidence.Cellular and molecular neurobiology · 2020Review
- Target-Derived Neurotrophic Factor Deprivation Puts Retinal Ganglion Cells on Death Row: Cold Hard Evidence and Caveats.International journal of molecular sciences · 2019Review
- Apoptotic cell death regulation in neurons.The FEBS journal · 2019Review
- Eye drop delivery of pigment epithelium-derived factor-34 promotes retinal ganglion cell neuroprotection and axon regeneration.Molecular and cellular neurosciences · 2015Article
- Neuritin 1 promotes retinal ganglion cell survival and axonal regeneration following optic nerve crush.Cell death & disease · 2015Article
- Role of Protease-Inhibitors in Ocular Diseases.Molecules (Basel, Switzerland) · 2014Review
- Upregulation of Gem relates to retinal ganglion cells apoptosis after optic nerve crush in adult rats.Journal of molecular histology · 2014Article
- Combined suppression of CASP2 and CASP6 protects retinal ganglion cells from apoptosis and promotes axon regeneration through CNTF-mediated JAK/STAT signalling.Brain : a journal of neurology · 2014Article
- Optic nerve crush induces spatial and temporal gene expression patterns in retina and optic nerve of BALB/cJ mice.Molecular neurodegeneration · 2014Article
- Pigment epithelium-derived factor is retinal ganglion cell neuroprotective and axogenic after optic nerve crush injury.Investigative ophthalmology & visual science · 2013Article
- What can we learn about stroke from retinal ischemia models?Acta pharmacologica Sinica · 2013Review
- Dose-dependent treatment of optic nerve crush by exogenous systemic mutant erythropoietin.Experimental eye research · 2012Article
- Pharmacological inhibition of caspase-2 protects axotomised retinal ganglion cells from apoptosis in adult rats.PloS one · 2012Article
- Involvement of caspase-6 and caspase-8 in neuronal apoptosis and the regenerative failure of injured retinal ganglion cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Calpain inhibition attenuates apoptosis of retinal ganglion cells in acute optic neuritis.Investigative ophthalmology & visual science · 2011Article
- Ocular neuroprotection by siRNA targeting caspase-2.Cell death & disease · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The majority of retinal ganglion cells (RGCs) degenerate and die after transection of the optic nerve (ON) in the adult rat. This secondary cell death can primarily be ascribed to apoptosis. Recent work strongly suggests a decisive role for a family of cysteine proteases, termed caspases, as mediators of neuronal apoptosis. In this study, we investigated whether activation of caspases contributes to delayed death of RGCs after axotomy. Intraocular application of various caspase inhibitors rescued up to 34% of RGCs that would otherwise have died 14 d after ON transection. Using a modified affinity-labeling technique, we detected a 17 kDa protease subunit upregulated after axotomy. Upregulation was prevented by caspase inhibitor treatment. The 17 kDa protein was identified as a CPP32-like protease by Western blot analysis and affinity labeling with biotinylated acetyl-Asp-Glu-Val-Asp-aldehyde, which specifically inhibits CPP32-like caspases. In vivo application of the irreversible caspase inhibitor benzyloxycarbonyl-Asp-Glu-Val-Asp-chloromethylketone revealed CPP32-like proteases to be major mediators of caspase-induced apoptosis in axotomized RGCs, because this inhibitor showed an even higher neuroprotective potential than the irreversible wide-range inhibitor benzyloxycarbonyl-Val-Ala-DL-Asp-fluoromethylketone. In summary, the data presented here provide further insight into the mechanisms of injury-induced neuronal apoptosis and could give rise to more effective therapeutic intervention strategies in CNS trauma and neurodegenerative diseases.
Indexed as
Identifiers
9614240PMC6792678W1760718049What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.