ArticleMolecular biology of the cell1998
Virulence and functions of myosin II are inhibited by overexpression of light meromyosin in Entamoeba histolytica.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 85 citations in OpenAlex.
- An atypical venus fly trap domain receptor regulates motility and phagocytosis in the protozoan parasite Entamoeba histolytica.PLoS pathogens · 2026Article
- Work with me here: variations in genome content and emerging genetic tools in Entamoeba histolytica.Trends in parasitology · 2025Review
- Fibronectin induces a transition from amoeboid to a fan morphology and modifies migration in Entamoeba histolytica.PLoS pathogens · 2024Article
- Pathogenicity and virulence ofVirulence · 2023Review
- Live Cells Imaging and Comparative Phosphoproteomics Uncover Proteins from the Mechanobiome inInternational journal of molecular sciences · 2023Article
- PTEN differentially regulates endocytosis, migration, and proliferation in the enteric protozoan parasite Entamoeba histolytica.PLoS pathogens · 2022Article
- Unraveling the relevance of the polyadenylation factor EhCFIm25 in Entamoeba histolytica through proteomic analysis.FEBS open bio · 2021Article
- Bioimage Analysis and Cell Motility.Patterns (New York, N.Y.) · 2021Review
- Advances inFrontiers in microbiology · 2019Review
- Morphodynamics of the Actin-Rich Cytoskeleton inFrontiers in cellular and infection microbiology · 2018Article
- Entamoeba histolytica: Host parasite interactions at the colonic epithelium.Tissue barriers · 2017Article
- The α-helical regions of KERP1 are important in Entamoeba histolytica adherence to human cells.Scientific reports · 2013Article
- Feed-forward regulation of phagocytosis by Entamoeba histolytica.Infection and immunity · 2012Article
- Heterotrimeric G-protein signaling is critical to pathogenic processes in Entamoeba histolytica.PLoS pathogens · 2012Article
- Localisation to lipid rafts correlates with increased function of the Gal/GalNAc lectin in the human protozoan parasite, Entamoeba histolytica.International journal for parasitology · 2011Article
- A proteomic and cellular analysis of uropods in the pathogen Entamoeba histolytica.PLoS neglected tropical diseases · 2011Article
- A C2 domain protein kinase initiates phagocytosis in the protozoan parasite Entamoeba histolytica.Nature communications · 2011Article
- Entamoeba histolytica encodes unique formins, a subset of which regulates DNA content and cell division.Infection and immunity · 2008Article
- Acto-myosin cytoskeleton dependent viscosity and shear-thinning behavior of the amoeba cytoplasm.European biophysics journal : EBJ · 2005Article
- Roles of cell adhesion and cytoskeleton activity in Entamoeba histolytica pathogenesis: a delicate balance.Infection and immunity · 2005Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several changes in cell morphology take place during the capping of surface receptors in Entamoeba histolytica. The amoebae develop the uroid, an appendage formed by membrane invaginations, which accumulates ligand-receptor complexes resulting from the capping process. Membrane shedding is particularly active in the uroid region and leads to the elimination of accumulated ligands. This appendage has been postulated to participate in parasitic defense mechanisms against the host immune response, because it eliminates complement and specific antibodies bound to the amoeba surface. The involvement of myosin II in the capping process of surface receptors has been suggested by experiments showing that drugs that affect myosin II heavy-chain phosphorylation prevent this activity. To understand the role of this mechanoenzyme in surface receptor capping, a myosin II dominant negative strain was constructed. This mutant is the first genetically engineered cytoskeleton-deficient strain of E. histolytica. It was obtained by overexpressing the light meromyosin domain, which is essential for myosin II filament formation. E. histolytica overexpressing light meromyosin domain displayed a myosin II null phenotype characterized by abnormal movement, failure to form the uroid, and failure to undergo the capping process after treatment with concanavalin A. In addition, the amoebic cytotoxic capacities of the transfectants on human colon cells was dramatically reduced, indicating a role for cytoskeleton in parasite pathogenicity.
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