ArticleGenetics1998
Probable mechanisms underlying interallelic complementation and temperature-sensitivity of mutations at the shibire locus of Drosophila melanogaster.
Article in Genetics, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- The shibireInsect science · 2026Article
- A candidate loss-of-function variant in SGIP1 causes synaptic dysfunction and recessive parkinsonism.Cell reports. Medicine · 2024Article
- Altered Grooming Cycles in Transgenic Drosophila.Behavior genetics · 2024Article
- Review
- Clathrin-independent pathways of endocytosis.Cold Spring Harbor perspectives in biology · 2014Article
- Dynamin photoinactivation blocks Clathrin and α-adaptin recruitment and induces bulk membrane retrieval.The Journal of cell biology · 2014Article
- Using translational enhancers to increase transgene expression in Drosophila.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- A role for dynamin in triggering ethanol tolerance.Alcoholism, clinical and experimental research · 2012Article
- Article
- Endocytosis unplugged: multiple ways to enter the cell.Cell research · 2010Review
- Overexpressing temperature-sensitive dynamin decelerates phototransduction and bundles microtubules in Drosophila photoreceptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- An internal GAP domain negatively regulates presynaptic dynamin in vivo: a two-step model for dynamin function.The Journal of cell biology · 2005Article
- Evidence for cell autonomous AP1 function in regulation of Drosophila motor-neuron plasticity.BMC neuroscience · 2003Article
- Regulation of dynamin by nucleoside diphosphate kinase.Journal of bioenergetics and biomembranes · 2003Review
- Effects of mutations at the stambh A locus of Drosophila melanogaster.Journal of genetics · 2001Article
- Disruption of neurotransmission in Drosophila mushroom body blocks retrieval but not acquisition of memory.Nature · 2001Article
- Drosophila stoned proteins regulate the rate and fidelity of synaptic vesicle internalization.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- A product of the Drosophila stoned locus regulates neurotransmitter release.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The shibire locus of Drosophila melanogaster encodes dynamin, a GTPase required for the fission of endocytic vesicles from plasma membrane. Biochemical studies indicate that mammalian dynamin is part of a complex containing multiple dynamin subunits and other polypeptides. To gain insight into sequences of dynamin critical for its function, we have characterized in detail a collection of conditional and lethal shi alleles. We describe a probable null allele of shi and show that its properties are distinct from those of two classes of lethal alleles (termed I and II) that show intergroup, interallelic complementation. Sequenced class I alleles, which display dominant properties, carry missense mutations in conserved residues in the GTPase domain of dynamin. In contrast, the sequenced class II alleles, which appear completely recessive, carry missense mutations in conserved residues of a previously uncharacterized "middle domain" that lies adjacent to the GTPase region. These data suggest that critical interactions mediated by this middle domain are severely affected by the class II lethal mutations; thus, the mutant sequences should be very useful for confirming the in vivo relevance of interactions observed in vitro. Viable heteroallelic combinations of shi lethals show rapid and reversible temperature-sensitive paralytic phenotypes hitherto only described for the ts alleles of shi. When taken together with the molecular analysis of shi mutations, these observations suggest that the GTPase domain of dynamin carries an intrinsically temperature-sensitive activity: hypomorphic mutations that reduce this activity at low temperatures result in conditional temperature-sensitive phenotype. These observations explain why screens for conditional paralytic mutants in Drosophila inevitably recover ts alleles of shi at high frequencies.
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