ArticleGenetics1998
Genetics of the deflagellation pathway in Chlamydomonas.
Article in Genetics, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 56 citations in OpenAlex.
- Shear-induced ciliary autotomy mediated by a TRP channel and a voltage-dependent calcium channel.iScience · 2025Article
- Assembly of FAP93 at the proximal axoneme in Chlamydomonas cilia.Cytoskeleton (Hoboken, N.J.) · 2024Article
- Shedding of ciliary vesicles at a glance.Journal of cell science · 2022Article
- Responses to transient receptor potential (TRP) channel agonists inBiology open · 2020Article
- MKS-NPHP module proteins control ciliary shedding at the transition zone.PLoS biology · 2020Article
- Developmental change in translation initiation alters the localization of a common microbial protein necessary for Toxoplasma chronic infection.Molecular microbiology · 2016Article
- A Forward Genetic Screen and Whole Genome Sequencing Identify Deflagellation Defective Mutants in Chlamydomonas, Including Assignment of ADF1 as a TRP Channel.G3 (Bethesda, Md.) · 2016Article
- A NIMA-related kinase, CNK4, regulates ciliary stability and length.Molecular biology of the cell · 2016Article
- The centrosome and its duplication cycle.Cold Spring Harbor perspectives in biology · 2015Review
- The NIMA-family kinase Nek3 regulates microtubule acetylation in neurons.Journal of cell science · 2009Article
- Katanin knockdown supports a role for microtubule severing in release of basal bodies before mitosis in Chlamydomonas.Molecular biology of the cell · 2009Article
- Cilia and the cell cycle?The Journal of cell biology · 2005Review
- A NIMA-related kinase, Fa2p, localizes to a novel site in the proximal cilia of Chlamydomonas and mouse kidney cells.Molecular biology of the cell · 2004Article
- PF15p is the chlamydomonas homologue of the Katanin p80 subunit and is required for assembly of flagellar central microtubules.Eukaryotic cell · 2004Article
- Chlamydomonas fla mutants reveal a link between deflagellation and intraflagellar transport.BMC cell biology · 2003Article
- A role for katanin-mediated axonemal severing during Chlamydomonas deflagellation.Molecular biology of the cell · 1998Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Signal-induced deflagellation in Chlamydomonas involves Ca2+-activated breakage of the nine outer-doublet axonemal microtubules at a specific site in the flagellar transition zone. In this study, we isolated 13 new deflagellation mutants that can be divided into two phenotypic classes, the Adf class and the Fa class. Cells with the Adf deflagellation phenotype are defective in acid-stimulated Ca2+ influx, but can be induced to deflagellate by treatment with nonionic detergent and Ca2+. Genetic analyses show that the five new Adf mutations, as well as the previously identified adf1 mutation, are alleles of the ADF1 gene. Mutants in the second phenotypic class, the Fa mutants, fail to deflagellate in response to any known chemical stimulus and are defective in Ca2+-activated microtubule severing. Genetic analysis of these eight new Fa strains demonstrated that they define two complementation groups, and one of these contains the previously identified fa1 mutation. Diploid analysis showed that five alleles map to the FA1 gene, whereas four alleles define a novel gene that we have named FA2. The isolation of multiple mutant alleles of each gene, generated by either ultraviolet irradiation or insertional mutagenesis, indicates that ADF1, FA1, and FA2 may be the only genes that can be identified in a loss-of-function screen. These alleles should provide a better understanding of the regulation of microtubule severing by Ca2+.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.