ArticleThe Journal of general physiology1998
Kinetic structure of large-conductance Ca2+-activated K+ channels suggests that the gating includes transitions through intermediate or secondary states. A mechanism for flickers.
Article in The Journal of general physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed.
- Structural basis of voltage-dependent gating in BK channels.Nature communications · 2025Article
- Markov models and long-term memory in ion channels: A contradiction in terms?Biophysical journal · 2025Article
- The conductance of KCNQ2 and its pathogenic variants is determined by individual subunit gating.Science advances · 2025Article
- A deep learning approach to real-time Markov modeling of ion channel gating.Communications chemistry · 2024Article
- HS-AFM single-molecule structural biology uncovers basis of transporter wanderlust kinetics.Nature structural & molecular biology · 2024Article
- How much does TRPV1 deviate from an ideal MWC-type protein?Biophysical journal · 2024Article
- Evaluating sequential and allosteric activation models in IKs channels with mutated voltage sensors.The Journal of general physiology · 2024Article
- Microscopic mechanism of PIEZO1 activation by pressure-induced membrane stretch.The Journal of general physiology · 2023Article
- 2D-dwell-time analysis with simulations of ion-channel gating using high-performance computing.Biophysical journal · 2023Article
- Regulatory mechanisms of mitochondrial BKChannels (Austin, Tex.) · 2021Review
- Permeating disciplines: Overcoming barriers between molecular simulations and classical structure-function approaches in biological ion transport.Biochimica et biophysica acta. Biomembranes · 2018Review
- Understanding the conformational motions of RCK gating rings.The Journal of general physiology · 2017Review
- Deletion of cytosolic gating ring decreases gate and voltage sensor coupling in BK channels.The Journal of general physiology · 2017Article
- Structural basis for gating the high-conductance CaNature · 2017Article
- Single-channel kinetics of BK (Slo1) channels.Frontiers in physiology · 2014Review
- Free-energy relationships in ion channels activated by voltage and ligand.The Journal of general physiology · 2013Article
- The BK channel: a vital link between cellular calcium and electrical signaling.Protein & cell · 2012Review
- Modulation of BK channel voltage gating by different auxiliary β subunits.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Short isoforms of the cold receptor TRPM8 inhibit channel gating by mimicking heat action rather than chemical inhibitors.The Journal of biological chemistry · 2012Article
- Voltage- and cold-dependent gating of single TRPM8 ion channels.The Journal of general physiology · 2011Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Mechanisms for the Ca2+-dependent gating of single large-conductance Ca2+-activated K+ channels from cultured rat skeletal muscle were developed using two-dimensional analysis of single-channel currents recorded with the patch clamp technique. To extract and display the essential kinetic information, the kinetic structure, from the single channel currents, adjacent open and closed intervals were binned as pairs and plotted as two-dimensional dwell-time distributions, and the excesses and deficits of the interval pairs over that expected for independent pairing were plotted as dependency plots. The basic features of the kinetic structure were generally the same among single large-conductance Ca2+-activated K+ channels, but channel-specific differences were readily apparent, suggesting heterogeneities in the gating. Simple gating schemes drawn from the Monod- Wyman-Changeux (MWC) model for allosteric proteins could approximate the basic features of the Ca2+ dependence of the kinetic structure. However, consistent differences between the observed and predicted dependency plots suggested that additional brief lifetime closed states not included in MWC-type models were involved in the gating. Adding these additional brief closed states to the MWC-type models, either beyond the activation pathway (secondary closed states) or within the activation pathway (intermediate closed states), improved the description of the Ca2+ dependence of the kinetic structure. Secondary closed states are consistent with the closing of secondary gates or channel block. Intermediate closed states are consistent with mechanisms in which the channel activates by passing through a series of intermediate conformations between the more stable open and closed states. It is the added secondary or intermediate closed states that give rise to the majority of the brief closings (flickers) in the gating.
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