ArticleThe Journal of cell biology1998
Microinjection of antibody to Mad2 protein into mammalian cells in mitosis induces premature anaphase.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
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Who cites it
86 citing papers in PubMed, 211 citations in OpenAlex.
- Differential roles for the spindle assembly checkpoint in error surveillance and mitotic timing.The EMBO journal · 2026Article
- A MOPD II-associated Pericentrin variant disrupts PACT domain dimerization and pericentriolar material recruitment.bioRxiv : the preprint server for biology · 2026Article
- CCDC68 Maintains Mitotic Checkpoint Activation by Promoting CDC20 Integration into the MCC.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Chromosomally unstable tumor cells specifically require KIF18A for proliferation.Nature communications · 2021Article
- Accessing Mitochondrial Protein Import in Living Cells by Protein Microinjection.Frontiers in cell and developmental biology · 2021Article
- Spindle Architectural Features Must Be Considered Along With Cell Size to Explain the Timing of Mitotic Checkpoint Silencing.Frontiers in physiology · 2020Article
- Manipulating cultured mammalian cells for mitosis research.Methods in cell biology · 2020Article
- A Gradient in Metaphase Tension Leads to a Scaled Cellular Response in Mitosis.Developmental cell · 2019Article
- HP1α targets the chromosomal passenger complex for activation at heterochromatin before mitotic entry.The EMBO journal · 2018Article
- Article
- Delayed APC/C activation extends the first mitosis of mouse embryos.Scientific reports · 2017Article
- Multiple Duties for Spindle Assembly Checkpoint Kinases in Meiosis.Frontiers in cell and developmental biology · 2017Review
- Chromosome missegregation during anaphase triggers p53 cell cycle arrest through histone H3.3 Ser31 phosphorylation.Nature cell biology · 2016Article
- Connecting the microtubule attachment status of each kinetochore to cell cycle arrest through the spindle assembly checkpoint.Chromosoma · 2015Review
- CENP-I and Aurora B act as a molecular switch that ties RZZ/Mad1 recruitment to kinetochore attachment status.The Journal of cell biology · 2014Article
- The spindle and kinetochore-associated (Ska) complex enhances binding of the anaphase-promoting complex/cyclosome (APC/C) to chromosomes and promotes mitotic exit.Molecular biology of the cell · 2014Article
- Probing the in vivo function of Mad1:C-Mad2 in the spindle assembly checkpoint.The EMBO journal · 2011Article
- polo Is Identified as a Suppressor of bubR1 Nondisjunction in a Deficiency Screen of the Third Chromosome in Drosophila melanogaster.G3 (Bethesda, Md.) · 2011Article
- Spindle assembly checkpoint regulates mitotic cell cycle progression during preimplantation embryo development.PloS one · 2011Article
- Effect of Mad2 on paclitaxel-induced cell death in ovarian cancer cells.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2010Article
26 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In yeast, the Mad2 protein is required for the M phase arrest induced by microtubule inhibitors, but the protein is not essential under normal culture conditions. We tested whether the Mad2 protein participates in regulating the timing of anaphase onset in mammalian cells in the absence of microtubule drugs. When microinjected into living prophase or prometaphase PtK1 cells, anti-Mad2 antibody induced the onset of anaphase prematurely during prometaphase, before the chromosomes had assembled at the metaphase plate. Anti-Mad2 antibody-injected cells completed all aspects of anaphase including chromatid movement to the spindle poles and pole-pole separation. Identical results were obtained when primary human keratinocytes were injected with anti-Mad2 antibody. These studies suggest that Mad2 protein function is essential for the timing of anaphase onset in somatic cells at each mitosis. Thus, in mammalian somatic cells, the spindle checkpoint appears to be a component of the timing mechanism for normal mitosis, blocking anaphase onset until all chromosomes are aligned at the metaphase plate.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.