ArticleApplied and environmental microbiology1998
Low-frequency horizontal transfer of an element containing the chlorocatechol degradation genes from Pseudomonas sp. strain B13 to Pseudomonas putida F1 and to indigenous bacteria in laboratory-scale activated-sludge microcosms.
Article in Applied and environmental microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 88 citations in OpenAlex.
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- Construction of synthetic anti-fouling consortia: fouling control effects and polysaccharide degradation mechanisms.Microbial cell factories · 2023Article
- Life Within a Contaminated Niche: Comparative Genomic Analyses of an Integrative Conjugative Element ICEFrontiers in microbiology · 2022Article
- Combinatorial pathway balancing provides biosynthetic access to 2-fluoro-Chem catalysis · 2021Article
- The role of mobile genetic elements in organic micropollutant degradation during biological wastewater treatment.Water research X · 2020Review
- Why? - Successful Pseudomonas aeruginosa clones with a focus on clone C.FEMS microbiology reviews · 2020Review
- Article
- The hidden life of integrative and conjugative elements.FEMS microbiology reviews · 2017Review
- Non-invasive determination of conjugative transfer of plasmids bearing antibiotic-resistance genes in biofilm-bound bacteria: effects of substrate loading and antibiotic selection.Applied microbiology and biotechnology · 2013Article
- Stability of a Pseudomonas putida KT2440 bacteriophage-carried genomic island and its impact on rhizosphere fitness.Applied and environmental microbiology · 2012Article
- Cellular variability of RpoS expression underlies subpopulation activation of an integrative and conjugative element.PLoS genetics · 2012Article
- Horizontal gene exchange in environmental microbiota.Frontiers in microbiology · 2011Article
- The accessory genome of Pseudomonas aeruginosa.Microbiology and molecular biology reviews : MMBR · 2010Review
- Units of plasticity in bacterial genomes: new insight from the comparative genomics of two bacteria interacting with invertebrates, Photorhabdus and Xenorhabdus.BMC genomics · 2010Article
- ICEEc2, a new integrative and conjugative element belonging to the pKLC102/PAGI-2 family, identified in Escherichia coli strain BEN374.Journal of bacteriology · 2010Article
- Integrative and conjugative elements: mosaic mobile genetic elements enabling dynamic lateral gene flow.Nature reviews. Microbiology · 2010Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The possibilities for low-frequency horizontal transfer of the self-transmissible chlorocatechol degradative genes (clc) from Pseudomonas sp. strain B13 were investigated in activated-sludge microcosms. When the clc genes were transferred into an appropriate recipient bacterium such as Pseudomonas putida F1, a new metabolic pathway for chlorobenzene degradation was formed by complementation which could be selected for by the addition of mono- or 1, 4-dichlorobenzene (CB). Under optimized conditions with direct donor-recipient filter matings, very low transfer frequencies were observed (approximately 3.5 x 10(-8) per donor per 24 h). In contrast, in matings on agar plate surfaces, transconjugants started to appear after 8 to 10 days, and their numbers then increased during prolonged continuous incubation with CB. In activated-sludge microcosms, CB-degrading (CB+) transconjugants of strain F1 which had acquired the clc genes were detected but only when strain B13 cell densities of more than 10(5) CFU/ml could be maintained by the addition of its specific growth substrate, 3-chlorobenzoate (3CBA). The CB+ transconjugants reached final cell densities of between 10(2) and 10(3) CFU/ml. When strain B13 was inoculated separately (without the designated recipient strain F1) into an activated-sludge microcosm, CB+ transconjugants could not be detected. However, in this case a new 3CBA-degrading strain appeared which had acquired the clc genes from strain B13. The effects of selective substrates on the survival and growth of and gene transfer between bacteria degrading aromatic pollutants in a wastewater ecosystem are discussed.
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