ArticleThe Biochemical journal1998
Characterization of sphingosine kinase (SK) activity in Saccharomyces cerevisiae and isolation of SK-deficient mutants.
Article in The Biochemical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 62 citations in OpenAlex.
- Article
- Toll-Like Receptor 4 Is Essential for the Expression of Sphingosine-1-Phosphate-Dependent Asthma-Like Disease in Mice.Frontiers in immunology · 2017Article
- Article
- Sphingolipids as cell fate regulators in lung development and disease.Apoptosis : an international journal on programmed cell death · 2015Review
- Autophagy in the light of sphingolipid metabolism.Apoptosis : an international journal on programmed cell death · 2015Review
- Sphingosine kinase A is a pleiotropic and essential enzyme for Leishmania survival and virulence.Molecular microbiology · 2013Article
- Sphingolipids: regulators of crosstalk between apoptosis and autophagy.Journal of lipid research · 2013Review
- Extracellular and intracellular actions of sphingosine-1-phosphate.Advances in experimental medicine and biology · 2010Review
- Coordination of the dynamics of yeast sphingolipid metabolism during the diauxic shift.Theoretical biology & medical modelling · 2007Article
- Arabidopsis sphingosine kinase and the effects of phytosphingosine-1-phosphate on stomatal aperture.Plant physiology · 2005Article
- Induction of apoptosis by sphingoid long-chain bases in Aspergillus nidulans.Molecular and cellular biology · 2003Article
- Human sphingosine kinase: purification, molecular cloning and characterization of the native and recombinant enzymes.The Biochemical journal · 2000Article
- Sli2 (Ypk1), a homologue of mammalian protein kinase SGK, is a downstream kinase in the sphingolipid-mediated signaling pathway of yeast.Molecular and cellular biology · 2000Article
- Sphingosine kinase expression increases intracellular sphingosine-1-phosphate and promotes cell growth and survival.The Journal of cell biology · 1999Article
- Analysis of phosphorylated sphingolipid long-chain bases reveals potential roles in heat stress and growth control in Saccharomyces.Journal of bacteriology · 1999Article
- Signal transduction of stress via ceramide.The Biochemical journal · 1998Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Sphingosine kinase (SK) catalyses the phosphorylation of sphingosine to generate sphingosine 1-phosphate, which is a second messenger involved in the proliferative responses of mammalian cells. Although the yeast Saccharomyces cerevisiae has similar phosphorylated sphingoid bases which appear to be involved in growth regulation and the response to stress, SK activity had not been previously demonstrated in yeast. In this study, an in vitro system was set up to characterize yeast SK activity. Activity was detected in the cytosol at neutral pH and 37 degreesC. Yeast SK phosphorylated the sphingoid bases sphingosine, dihydrosphingosine and phytosphingosine. (d,l)-threo-dihydrosphingosine, an inhibitor of mammalian SK, did not inhibit the yeast enzyme. Unique properties of yeast SK were an optimal temperature of 43 degreesC, and in vivo activation during nutrient deprivation. Spontaneous mutants with diminished SK activity were isolated utilizing a screen for resistance to sphingosine in a sphingosine-phosphate-lyase deletion background. Abnormal growth and heat sensitivity were observed in these mutants. These findings suggest that SK may function as a stress-response protein in yeast.
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