ArticleProceedings of the National Academy of Sciences of the United States of America1998
Water does not flow across the tight junctions of MDCK cell epithelium.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 83 citations in OpenAlex.
- Regulation of epithelial tissue homeostasis by active transepithelial transport.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Cell-Electrode Models for Impedance Analysis of Epithelial and Endothelial Monolayers Cultured on Microelectrodes.Sensors (Basel, Switzerland) · 2024Article
- ILDR1 is important for paracellular water transport and urine concentration mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Mechanical Characterization of Microengineered Epithelial Cysts by Using Atomic Force Microscopy.Biophysical journal · 2017Article
- Claudins in barrier and transport function-the kidney.Pflugers Archiv : European journal of physiology · 2017Review
- Article
- The Sodium Glucose Cotransporter SGLT1 Is an Extremely Efficient Facilitator of Passive Water Transport.The Journal of biological chemistry · 2016Article
- A spatial model of fluid recycling in the airways of the lung.Journal of theoretical biology · 2015Article
- Direct visualization and quantitative analysis of water diffusion in complex biological tissues using CARS microscopy.Scientific reports · 2013Article
- Measuring collective cell movement and extracellular matrix interactions using magnetic resonance imaging.Scientific reports · 2013Article
- Collecting duct cells that lack normal cilia have mislocalized vasopressin-2 receptors.American journal of physiology. Renal physiology · 2012Article
- Osmoregulation and epithelial water transport: lessons from the intestine of marine teleost fish.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2012Review
- Monitoring single-channel water permeability in polarized cells.The Journal of biological chemistry · 2011Article
- Shiga toxin is transported into the nucleoli of intestinal epithelial cells via a carrier-dependent process.Toxins · 2010Article
- The permeability of SPION over an artificial three-layer membrane is enhanced by external magnetic field.Journal of nanobiotechnology · 2006Article
- A new approach to epithelial isotonic fluid transport: an osmosensor feedback model.The Journal of membrane biology · 2006Article
- The Role of the Tight Junction in Paracellular Fluid Transport across Corneal Endothelium. Electro-osmosis as a Driving Force.The Journal of membrane biology · 2006Review
- Na+ recirculation and isosmotic transport.The Journal of membrane biology · 2006Review
- Cyclic AMP is sufficient for triggering the exocytic recruitment of aquaporin-2 in renal epithelial cells.EMBO reports · 2003Article
- Hypotonic treatment evokes biphasic ATP release across the basolateral membrane of cultured renal epithelia (A6).The Journal of physiology · 2002Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although it has been known for decades that the tight junctions of fluid-transporting epithelia are leaky to ions, it has not been possible to determine directly whether significant transjunctional water movement also occurs. An optical microscopic technique was developed for the direct visualization of the flow velocity profiles within the lateral intercellular spaces of a fluid-absorptive, cultured renal epithelium (MDCK) and used to determine the velocity of the fluid flow across the tight junction. The flow velocity within the lateral intercellular spaces fell to near zero adjacent to the tight junction, showing that significant transjunctional flow did not occur, even when transepithelial fluid movement was augmented by imposition of osmotic gradients.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.