ArticleProceedings of the National Academy of Sciences of the United States of America1998
Does the colonic H,K-ATPase also act as an Na,K-ATPase?
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 79 citations in OpenAlex.
- Airway Surface Liquid pH Regulation in Airway Epithelium Current Understandings and Gaps in Knowledge.International journal of molecular sciences · 2021Review
- Increased colonic KScientific reports · 2021Article
- Increased expression of ATP12A proton pump in cystic fibrosis airways.JCI insight · 2018Article
- Iron is a substrate of theThe Journal of biological chemistry · 2017Article
- Retinoschisin is linked to retinal Na/K-ATPase signaling and localization.Molecular biology of the cell · 2017Article
- The renal cortical collecting duct: a secreting epithelium?The Journal of physiology · 2016Article
- P2C-Type ATPases and Their Regulation.Molecular neurobiology · 2016Review
- Parameter estimation for mathematical models of a nongastric H+(Na+)-K(+)(NH4+)-ATPase.American journal of physiology. Renal physiology · 2015Article
- Molecular mechanisms and regulation of urinary acidification.Comprehensive Physiology · 2014Review
- A link between fertility and K+ homeostasis: role of the renal H,K-ATPase type 2.Pflugers Archiv : European journal of physiology · 2013Article
- The parietal cell gastric H, K-ATPase also functions as the Na, K-ATPase and Ca-ATPase in altered states.F1000Research · 2013Article
- Deceleration of the E1P-E2P transition and ion transport by mutation of potentially salt bridge-forming residues Lys-791 and Glu-820 in gastric H+/K+-ATPase.The Journal of biological chemistry · 2010Article
- Discovery and validation of colonic tumor-associated proteins via metabolic labeling and stable isotopic dilution.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- The non-gastric H,K-ATPase as a tool to study the ouabain-binding site in Na,K-ATPase.Pflugers Archiv : European journal of physiology · 2009Article
- Palytoxin acts on Na(+),K (+)-ATPase but not nongastric H(+),K (+)-ATPase.The Journal of membrane biology · 2007Article
- Molecular identification of Sch28080-sensitive K-ATPase activities in the mouse kidney.Pflugers Archiv : European journal of physiology · 2006Article
- CD63 interacts with the carboxy terminus of the colonic H+-K+-ATPase to decrease [corrected] plasma membrane localization and 86Rb+ uptake.American journal of physiology. Cell physiology · 2005Article
- Novel p-type ATPases mediate high-affinity potassium or sodium uptake in fungi.Eukaryotic cell · 2004Article
- The functional role of beta subunits in oligomeric P-type ATPases.Journal of bioenergetics and biomembranes · 2001Review
- A transmembrane segment determines the steady-state localization of an ion-transporting adenosine triphosphatase.The Journal of cell biology · 2000Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously have demonstrated that the colonic P-ATPase alpha subunit cDNA encodes an H,K-ATPase when expressed in Xenopus laevis oocytes. Besides its high level of amino acid homology (75%) with the Na,K-ATPase, the colonic H,K-ATPase also shares a common pharmacological profile with Na,K-ATPase, because both are ouabain-sensitive and Sch 28080-insensitive. These features raise the possibility that an unrecognized property of the colonic H, K-ATPase would be Na+ translocation. To test this hypothesis, ion-selective microelectrodes were used to measure the intracellular Na+ activity of X. laevis oocytes expressing various combinations of P-ATPase subunits. The results show that expression in oocytes of the colonic H,K-ATPase affects intracellular Na+ homeostasis in a way similar to the expression of the Bufo marinus Na,K-ATPase; intracellular Na+ activity is lower in oocytes expressing the colonic H,K-ATPase or the B. marinus Na,K-ATPase than in oocytes expressing the gastric H,K-ATPase or a beta subunit alone. In oocytes expressing the colonic H,K-ATPase, the decrease in intracellular Na+ activity persists when diffusive Na+ influx is enhanced by functional expression of the amiloride-sensitive epithelial Na+ channel, suggesting that the decrease is related to increased active Na+ efflux. The Na+ decrease depends on the presence of K+ in the external medium and is inhibited by 2 mM ouabain, a concentration that inhibits the colonic H,K-ATPase. These data are consistent with the hypothesis that the colonic H,K-ATPase may transport Na+, acting as an (Na,H),K-ATPase. Despite its molecular and functional characterization, the physiological role of the colonic (Na,H),K-ATPase in colonic and renal ion homeostasis remains to be elucidated.
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