ArticleProceedings of the National Academy of Sciences of the United States of America1998
Nonneuronal isoforms of STOP protein are responsible for microtubule cold stability in mammalian fibroblasts.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 59 citations in OpenAlex.
- Reevaluating tau reduction as a therapeutic approach for tauopathies: Insights and perspectives.Cytoskeleton (Hoboken, N.J.) · 2024Article
- Calcium entry units (CEUs): perspectives in skeletal muscle function and disease.Journal of muscle research and cell motility · 2021Article
- Temperature-Induced uncoupling of cell cycle regulators.Developmental biology · 2021Article
- Beyond Neuronal Microtubule Stabilization: MAP6 and CRMPS, Two Converging Stories.Frontiers in molecular neuroscience · 2021Article
- Deletion of the microtubule-associated protein 6 (MAP6) results in skeletal muscle dysfunction.Skeletal muscle · 2018Article
- MAP6 interacts with Tctex1 and CaThe European journal of neuroscience · 2017Article
- Parvulin 17-catalyzed Tubulin Polymerization Is Regulated by Calmodulin in a Calcium-dependent Manner.The Journal of biological chemistry · 2015Article
- Article
- Structural basis for the association of MAP6 protein with microtubules and its regulation by calmodulin.The Journal of biological chemistry · 2013Article
- MAP6-F is a temperature sensor that directly binds to and protects microtubules from cold-induced depolymerization.The Journal of biological chemistry · 2012Article
- Cold exposure reveals two populations of microtubules in pulmonary endothelia.American journal of physiology. Lung cellular and molecular physiology · 2011Article
- Abnormal nociception and opiate sensitivity of STOP null mice exhibiting elevated levels of the endogenous alkaloid morphine.Molecular pain · 2010Article
- Review of the multiple aspects of neurofilament functions, and their possible contribution to neurodegeneration.Molecular neurobiology · 2008Review
- Deletion of the STOP gene, a microtubule stabilizing factor, leads only to discrete cerebral metabolic changes in mice.Journal of neuroscience research · 2008Article
- The suppression of brain cold-stable microtubules in mice induces synaptic defects associated with neuroleptic-sensitive behavioral disorders.Genes & development · 2002Article
- Two related subpellicular cytoskeleton-associated proteins in Trypanosoma brucei stabilize microtubules.Molecular biology of the cell · 2002Article
- Stepwise reconstitution of interphase microtubule dynamics in permeabilized cells and comparison to dynamic mechanisms in intact cells.The Journal of cell biology · 1998Article
- STOP proteins are responsible for the high degree of microtubule stabilization observed in neuronal cells.The Journal of cell biology · 1998Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
A number of cycling mammalian cells, such as NIH 3T3, contain abundant subsets of cold-stable microtubules. The origin of such microtubule stabilization in nonneuronal cells is unknown. We have previously described a neuronal protein, stable tubule-only polypeptide (STOP), that binds to microtubules and induces cold stability. We find that NIH 3T3 fibroblasts contain a major 42-kDa isoform of STOP (fibroblastic STOP, F-STOP). F-STOP contains the central repeats characteristic of brain STOP but shows extensive deletions of N- and C-terminal protein domains that are present in brain STOP. These deletions arise from differences in STOP RNA splicing. Despite such deletions, F-STOP has full microtubule stabilizing activity. F-STOP accumulates on cold-stable microtubules of interphase arrays and is present on stable microtubules within the mitotic spindle of NIH 3T3 cells. STOP inhibition by microinjection of affinity-purified STOP central repeat antibodies into NIH 3T3 cells abolishes both interphase and spindle microtubule cold stability. Similar results were obtained with Rat2 cells. These results show that STOP proteins have nonneuronal isoforms that are responsible for the microtubule cold stability observed in mammalian fibroblasts.
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