ArticleInfection and immunity1998
Modification of Ras in eukaryotic cells by Pseudomonas aeruginosa exoenzyme S.
Article in Infection and immunity, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 74 citations in OpenAlex.
- Individual contributions of exotoxins S and T on internalizedInfection and immunity · 2026Article
- Intracellular replication ofmSphere · 2023Article
- Intracellular replication of Pseudomonas aeruginosa in epithelial cells requires suppression of the caspase-4 inflammasome.bioRxiv : the preprint server for biology · 2023Article
- Exotoxin S secreted by internalized Pseudomonas aeruginosa delays lytic host cell death.PLoS pathogens · 2022Article
- Pharmacological activation of Rap1 antagonizes the endothelial barrier disruption induced by exotoxins ExoS and ExoT of Pseudomonas aeruginosa.Infection and immunity · 2015Article
- Sequential inactivation of Rho GTPases and Lim kinase by Pseudomonas aeruginosa toxins ExoS and ExoT leads to endothelial monolayer breakdown.Cellular and molecular life sciences : CMLS · 2014Article
- The impact of simvastatin on pulmonary effectors of Pseudomonas aeruginosa infection.PloS one · 2014Article
- Regulation of Rab5 function during phagocytosis of live Pseudomonas aeruginosa in macrophages.Infection and immunity · 2013Article
- Metastatic MTLn3 and non-metastatic MTC adenocarcinoma cells can be differentiated by Pseudomonas aeruginosa.Biology open · 2013Article
- The role of type III secretion system 2 in Vibrio parahaemolyticus pathogenicity.Journal of microbiology (Seoul, Korea) · 2012Review
- The role of potassium in inflammasome activation by bacteria.The Journal of biological chemistry · 2010Article
- ExoS controls the cell contact-mediated switch to effector secretion in Pseudomonas aeruginosa.Journal of bacteriology · 2008Article
- Metabolic regulation of type III secretion gene expression in Pseudomonas aeruginosa.Molecular microbiology · 2006Article
- Examination of the coordinate effects of Pseudomonas aeruginosa ExoS on Rac1.Infection and immunity · 2005Article
- Overexpression of the multidrug efflux pumps MexCD-OprJ and MexEF-OprN is associated with a reduction of type III secretion in Pseudomonas aeruginosa.Journal of bacteriology · 2005Article
- Characterization of an ExoS Type III translocation-resistant cell line.Infection and immunity · 2005Article
- Characterization of Pseudomonas aeruginosa exoenzyme S as a bifunctional enzyme in J774A.1 macrophages.Infection and immunity · 2003Article
- Exoenzyme S shows selective ADP-ribosylation and GTPase-activating protein (GAP) activities towards small GTPases in vivo.The Biochemical journal · 2002Article
- Membrane localization contributes to the in vivo ADP-ribosylation of Ras by Pseudomonas aeruginosa ExoS.Infection and immunity · 2002Article
- The human pathogen Pseudomonas aeruginosa utilizes conserved virulence pathways to infect the social amoeba Dictyostelium discoideum.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Genetic and functional data suggest that Pseudomonas aeruginosa exoenzyme S (ExoS), an ADP-ribosyltransferase, is translocated into eukaryotic cells by a bacterial type III secretory mechanism activated by contact between bacteria and host cells. Although purified ExoS is not toxic to eukaryotic cells, ExoS-producing bacteria cause reduced proliferation and viability, possibly mediated by bacterially translocated ExoS. To investigate the activity of translocated ExoS, we examined in vivo modification of Ras, a preferred in vitro substrate. The ExoS-producing strain P. aeruginosa 388 and an isogenic mutant strain, 388DeltaexoS, which fails to produce ExoS, were cocultured with HT29 colon carcinoma cells. Ras was found to be ADP-ribosylated during coculture with 388 but not with 388DeltaexoS, and Ras modification by 388 corresponded with reduction in HT29 cell DNA synthesis. Active translocation by bacteria was found to be required, since exogenous ExoS, alone or in the presence of 388DeltaexoS, was unable to modify intracellular Ras. Other ExoS-producing strains caused modification of Ras, indicating that this is not a strain-specific event. ADP-ribosylation of Rap1, an additional Ras family substrate for ExoS in vitro, was not detectable in vivo under conditions sufficient for Ras modification, suggesting possible ExoS substrate preference among Ras-related proteins. These results confirm that intracellular Ras is modified by bacterially translocated ExoS and that the inhibition of target cell proliferation correlates with the efficiency of Ras modification.
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