ArticleThe Plant cell1998
Barley aleurone cells contain two types of vacuoles. Characterization Of lytic organelles by use of fluorescent probes
Article in The Plant cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 174 citations in OpenAlex.
- Structural insights into how vacuolar sorting receptor recognizes the C-terminal sorting determinant of a vicilin-like seed storage protein.The FEBS journal · 2026Article
- Distinction of chia varieties in vivo and in vitro based on the flow cytometry and rosmarinic acid production.Applied microbiology and biotechnology · 2024Article
- Anthocyanin in the Vacuole of Red Onion Epidermal Cells Quenches Other Fluorescent Molecules.Plants (Basel, Switzerland) · 2019Article
- Vacuolar membrane structures and their roles in plant-pathogen interactions.Plant molecular biology · 2019Review
- Peg Biology: Deciphering the Molecular Regulations Involved During Peanut Peg Development.Frontiers in plant science · 2019Review
- Identification of novel macropinocytosis inhibitors using a rational screen of Food and Drug Administration-approved drugs.British journal of pharmacology · 2018Article
- Instability of endosperm development in amphiploids and their parental species in the genus Avena L.Plant cell reports · 2018Article
- Novel fluorochromes label tonoplast in living plant cells and reveal changes in vacuolar organization after treatment with protein phosphatase inhibitors.Protoplasma · 2018Article
- Asymmetric cell division and its role in cell fate determination in the green alga Tetraselmis indica.Journal of biosciences · 2015Article
- Preparation of silica nanoparticles loaded with nootropics and their in vivo permeation through blood-brain barrier.BioMed research international · 2015Article
- Senescence-Associated Vacuoles, a Specific Lytic Compartment for Degradation of Chloroplast Proteins?Plants (Basel, Switzerland) · 2014Review
- Differentiation mechanism and function of the cereal aleurone cells and hormone effects on them.Plant cell reports · 2014Review
- Live Cell Imaging During Germination Reveals Dynamic Tubular Structures Derived from Protein Storage Vacuoles of Barley Aleurone Cells.Plants (Basel, Switzerland) · 2014Article
- Fusion, rupture, and degeneration: the fate of in vivo-labelled PSVs in developing barley endosperm.Journal of experimental botany · 2014Article
- The dynamic behavior of storage organelles in developing cereal seeds and its impact on the production of recombinant proteins.Frontiers in plant science · 2014Review
- Links between ER stress and autophagy in plants.Plant signaling & behavior · 2013Review
- The Arabidopsis Na+/H+ antiporters NHX1 and NHX2 control vacuolar pH and K+ homeostasis to regulate growth, flower development, and reproduction.The Plant cell · 2011Article
- New insights into globoids of protein storage vacuoles in wheat aleurone using synchrotron soft X-ray microscopy.Journal of experimental botany · 2011Article
- From signal transduction to autophagy of plant cell organelles: lessons from yeast and mammals and plant-specific features.Protoplasma · 2010Review
- pH-sensitivity of YFP provides an intracellular indicator of programmed cell death.Plant methods · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Light microscopy was used to study the structure and function of vacuoles in living protoplasts of barley (Hordeum vulgare cv Himalaya) aleurone. Light microscopy showed that aleurone protoplasts contain two distinct types of vacuole: the protein storage vacuole and a lysosome-like organelle, which we have called the secondary vacuole. Fluorescence microscopy using pH-sensitive fluorescent probes and a fluorogenic substrate for cysteine proteases showed that both protein storage vacuoles and secondary vacuoles are acidic, lytic organelles. Ratio imaging showed that the pH of secondary vacuoles was lower in aleurone protoplasts incubated in gibberellic acid than in those incubated in abscisic acid. Uptake of fluorescent probes into intact, isolated protein storage vacuoles and secondary vacuoles required ATP and occurred via at least two types of vanadate-sensitive, ATP-dependent tonoplast transporters. One transporter catalyzed the accumulation of glutathione-conjugated probes, and another transported probes not conjugated to glutathione.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.