ArticleNucleic acids research1998
Sequence and expression characteristics of a nuclear-encoded chloroplast sigma factor from mustard (Sinapis alba).
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 59 citations in OpenAlex.
- Plastid Gene Transcription: An Update on Promoters and RNA Polymerases.Methods in molecular biology (Clifton, N.J.) · 2021Article
- SIG1, a sigma factor for the chloroplast RNA polymerase, differently associates with multiple DNA regions in the chloroplast chromosomes in vivo.International journal of molecular sciences · 2012Article
- Plant sigma factors and their role in plastid transcription.Plant cell reports · 2007Review
- Chlamydomonas reinhardtii encodes a single sigma70-like factor which likely functions in chloroplast transcription.Current genetics · 2006Article
- Molecular genetic analysis of chloroplast gene promoters dependent on SIG2, a nucleus-encoded sigma factor for the plastid-encoded RNA polymerase, in Arabidopsis thaliana.Nucleic acids research · 2003Article
- AtSig5 is an essential nucleus-encoded Arabidopsis sigma-like factor.Plant physiology · 2003Article
- Characterization of Arabidopsis plastid sigma-like transcription factors SIG1, SIG2 and SIG3.Plant molecular biology · 2003Article
- Unique architecture of the plastid ribosomal RNA operon promoter recognized by the multisubunit RNA polymerase in tobacco and other higher plants.The Plant cell · 2003Article
- Involvement of a nuclear-encoded basic helix-loop-helix protein in transcription of the light-responsive promoter of psbD.Plant physiology · 2001Article
- Requirement for cytoplasmic protein synthesis during circadian peaks of transcription of chloroplast-encoded genes in Chlamydomonas.Plant molecular biology · 2000Article
- Complementary expression of two plastid-localized sigma-like factors in maize.Plant physiology · 2000Article
- Identification of two essential sequence elements in the nonconsensus type II PatpB-290 plastid promoter by using plastid transcription extracts from cultured tobacco BY-2 cells.The Plant cell · 1999Article
- Functional analysis of two maize cDNAs encoding T7-like RNA polymerases.The Plant cell · 1999Article
- In vitro characterization of the tobacco rpoB promoter reveals a core sequence motif conserved between phage-type plastid and plant mitochondrial promoters.The EMBO journal · 1999Article
- Circadian-regulated transcription of the psbD light-responsive promoter in wheat chloroplasts.Plant physiology · 1998Article
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Plant chloroplasts contain transcription factors that functionally resemble bacterial sigma factors. We have cloned the full-length cDNA from mustard (Sinapis alba) for a 53 kDa derived polypeptide that contains similarity to regions 1.2-4.2 of sigma70-type factors. The amino acid sequence at the N-terminus has characteristics of a chloroplast transit peptide. An in vitro synthesized polypeptide containing this region was shown to be imported into the chloroplast and processed. The recombinant factor lacking the N-terminal extension was expressed in Escherichia coli and purified. It confers the ability on E.coli core RNA polymerase to bind specifically to a DNA fragment that contains the chloroplast psbA promoter. Transcription of the psbA template by E.coli core enzyme in the presence of recombinant SIG1 results in enhanced formation of transcripts of the size expected for correct initiation at the in vivo start site. Together, these data suggest that the mature protein acts as one of the chloroplast transcription factors in mustard. RNA gel blot hybridization reveals a transcript at approximately 1.8 kb, which is more abundant in light-grown than in dark-grown mustard seedlings.
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