ArticleNucleic acids research1998
DNA binding and transcription activation specificity of hepatocyte nuclear factor 4.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 60 citations in OpenAlex.
- Transcription factor binding site orientation and order are major drivers of gene regulatory activity.Nature communications · 2023Article
- Circulating Proteins Associated with Response and Resistance to Neoadjuvant Chemotherapy in HER2-Positive Breast Cancer.Cancers · 2022Article
- Allele-specific enhancers mediate associations between LCAT and ABCA1 polymorphisms and HDL metabolism.PloS one · 2019Article
- Apolipoproteins as Differentiating and Predictive Markers for Assessing Clinical Outcomes in Patients with Small Cell Lung Cancer.Yonsei medical journal · 2016Article
- Nuclear effects of G-protein receptor kinase 5 on histone deacetylase 5-regulated gene transcription in heart failure.Circulation. Heart failure · 2011Article
- Farnesoid X receptor represses hepatic human APOA gene expression.The Journal of clinical investigation · 2011Article
- Modification in oxidative stress, inflammation, and lipoprotein assembly in response to hepatocyte nuclear factor 4alpha knockdown in intestinal epithelial cells.The Journal of biological chemistry · 2010Article
- Molecular cloning and characterization of the human PED/PEA-15 gene promoter reveal antagonistic regulation by hepatocyte nuclear factor 4alpha and chicken ovalbumin upstream promoter transcription factor II.The Journal of biological chemistry · 2008Article
- Direct inhibition of the DNA-binding activity of POU transcription factors Pit-1 and Brn-3 by selective binding of a phenyl-furan-benzimidazole dication.Nucleic acids research · 2008Article
- Transcriptional regulation of the fucosyltransferase VI gene in hepatocellular carcinoma cells.Glycoconjugate journal · 2008Article
- Transcription factor network downstream of protease activated receptors (PARs) modulating mouse bladder inflammation.BMC immunology · 2007Article
- Negative autoregulation of HNF-4alpha gene expression by HNF-4alpha1.The Biochemical journal · 2005Article
- Distinct modes of regulation of transcription of hepatitis B virus by the nuclear receptors HNF4alpha and COUP-TF1.Journal of virology · 2003Article
- Co-operation of the transcription factor hepatocyte nuclear factor-4 with Sp1 or Sp3 leads to transcriptional activation of the human haem oxygenase-1 gene promoter in a hepatoma cell line.The Biochemical journal · 2002Article
- Activation of glucokinase gene expression by hepatic nuclear factor 4alpha in primary hepatocytes.The Biochemical journal · 2002Article
- Critical roles of nuclear receptor response elements in replication of hepatitis B virus.Journal of virology · 2001Article
- Regulation of cytochrome P450 (CYP) genes by nuclear receptors.The Biochemical journal · 2000Review
- Identification and characterization of cis-acting elements conferring insulin responsiveness on hamster cholesterol 7alpha-hydroxylase gene promoter.The Biochemical journal · 2000Article
- The activity of the activation function 2 of the human hepatocyte nuclear factor 4 (HNF-4alpha) is differently modulated by F domains from various origins.The Biochemical journal · 1999Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hepatocyte nuclear factor 4 (HNF-4) is an orphan intracellular receptor that appears to be a key factor in the regulation of energy metabolism. In order to gain greater understanding of the binding and activation requirements of HNF-4, we performed genetic analysis of the apoCIII promoter, a promoter that has previously been shown to be highly sensitive to HNF-4-induced transcription. We identified two elements within the apoCIII promoter that bind HNF-4, either of which are sufficient to confer promoter induction in response to HNF-4. These two elements are both direct repeat-like in nature, but they differ significantly in motif sequence and the repeats are separated by either 1 or 2 nt. Therefore, to better define the DNA sequence recognition requirements of HNF-4, we utilized PCR-based binding site selection. HNF-4 selected for direct repeat-like elements with either 1 or 2 nt between the repeats. Surprisingly, the strongest selection was for a core motif that included the nucleotides between the repeats. Mutation of the nucleotide between the repeats resulted in a 6-fold reduction in affinity, indicating that the interaction between HNF-4 and the intervening nucleotide(s) is critical for high affinity binding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.