ArticleNucleic acids research1998
The (6-4) photoproduct of thymine-thymine induces targeted substitution mutations in mammalian cells.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 73 citations in OpenAlex.
- MAPLE: a machine-learning force-field-native platform for automated reaction modeling and enzyme design.Chemical science · 2026Article
- Far-infrared irradiation attenuates vessel contraction by activating SERCA2 through disruption of SERCA2 and PLN interaction.PloS one · 2025Article
- A Comprehensive Assessment of Ultraviolet-Radiation-Induced Mutations inJournal of fungi (Basel, Switzerland) · 2024Article
- Article
- Pterygium-The Good, the Bad, and the Ugly.Cells · 2021Review
- Processing of a single ribonucleotide embedded into DNA by human nucleotide excision repair and DNA polymerase η.Scientific reports · 2019Article
- Analysis of large deletion mutations induced by abasic site analog in human cells.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2018Article
- Prevention of Photocarcinogenesis by Agonists of 5-HT1A and Antagonists of 5-HT2A Receptors.Molecular neurobiology · 2016Review
- An unexpected deamination reaction after hydrolysis of the pyrimidine (6-4) pyrimidone photoproduct.Organic letters · 2014Article
- High frequency of PTEN mutations in nevi and melanomas from xeroderma pigmentosum patients.Pigment cell & melanoma research · 2014Article
- Turn-on DNA damage sensors for the direct detection of 8-oxoguanine and photoproducts in native DNA.Journal of the American Chemical Society · 2011Article
- Article
- Biological properties of single chemical-DNA adducts: a twenty year perspective.Chemical research in toxicology · 2008Review
- Structural study of DNA duplexes containing the (6-4) photoproduct by fluorescence resonance energy transfer.Nucleic acids research · 2001Article
- Error-prone lesion bypass by human DNA polymerase eta.Nucleic acids research · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two major ultraviolet-induced photolesions of TpT, a (6-4) photoproduct [T(6-4)T] and a cis-syn cyclobutane TT dimer (T=T), were incorporated into a predetermined site of one of the leading and lagging template strands of a double-stranded vector, and the modified DNAs were transfected into simian COS-7 cells. The DNAs replicated in the cells were recovered and were transfected again into Escherichia coli. The DNA replication efficiencies of plasmids containing T(6-4)T and T=T in the template strand for lagging strand synthesis were 93 and 79%, respectively, as compared with the unmodified DNA. Similar inhibitory effects were observed in the cases of the photoproducts in the template strand for leading strand synthesis (71 and 58%, respectively). These results indicated that T(6-4)T blocked DNA replication more weakly than T=T during leading and lagging strand syntheses in mammalian cells. The mutation frequencies of T(6-4)T were 2.3 and 4.7% in the leading and lagging template strands, respectively. The T=T lesion was less mutagenic and induced mutations with 0.2-0.7% frequencies. The T(6-4)T lesion primarily elicited 3'-T-->C substitutions, and T=T induced various types of mutations. These results indicate that T(6-4)T is more mutagenic than T=T during leading and lagging strand syntheses in simian cells. Moreover, this is the first evidence that shows T(6-4)T mainly elicits targeted substitutions at its 3'-T site in mammalian cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.