ArticleNucleic acids research1998
The environment of 5S rRNA in the ribosome: cross-links to the GTPase-associated area of 23S rRNA.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 48 citations in OpenAlex.
- Current advances of carbene-mediated photoaffinity labeling in medicinal chemistry.RSC advances · 2018Review
- Profiling of 2'-O-Me in human rRNA reveals a subset of fractionally modified positions and provides evidence for ribosome heterogeneity.Nucleic acids research · 2016Article
- General approach for introduction of various chemical labels in specific RNA locations based on insertion of amino linkers.Molecules (Basel, Switzerland) · 2013Review
- Bioorthogonal labeling of 5-hydroxymethylcytosine in genomic DNA and diazirine-based DNA photo-cross-linking probes.Accounts of chemical research · 2011Article
- A diazirine-based nucleoside analogue for efficient DNA interstrand photocross-linking.Journal of the American Chemical Society · 2008Article
- Changes in the conformation of 5S rRNA cause alterations in principal functions of the ribosomal nanomachine.Nucleic acids research · 2007Article
- 5S rRNA: Structure and Function from Head to Toe.International journal of biomedical science : IJBS · 2005Article
- 5S rRNA: Structure and Function from Head to Toe.International journal of biomedical science : IJBS · 2005Article
- 5 S rRNA: structure and interactions.The Biochemical journal · 2003Review
- Detailed analysis of RNA-protein interactions within the bacterial ribosomal protein L5/5S rRNA complex.RNA (New York, N.Y.) · 2002Article
- Mining biochemical information: lessons taught by the ribosome.RNA (New York, N.Y.) · 2002Article
- Saturation mutagenesis of 5S rRNA in Saccharomyces cerevisiae.Molecular and cellular biology · 2001Article
- Initiation factor IF 2 binds to the alpha-sarcin loop and helix 89 of Escherichia coli 23S ribosomal RNA.RNA (New York, N.Y.) · 2001Article
- Two crystal forms of helix II of Xenopus laevis 5S rRNA with a cytosine bulge.RNA (New York, N.Y.) · 2000Article
- Assembly of 5S ribosomal RNA is required at a specific step of the pre-rRNA processing pathway.The Journal of cell biology · 1999Article
- The NMR structure of Escherichia coli ribosomal protein L25 shows homology to general stress proteins and glutaminyl-tRNA synthetases.The EMBO journal · 1998Article
- Contacts of elongation factor G with the small ribosomal subunit: cross-linking approach.Doklady. Biochemistry and biophysicsArticle
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two photoreactive diazirine derivatives of uridine were used to study contacts between 5S rRNA and 23 rRNA in situ in Escherichia coli ribosomes. 2'-Amino-2'-deoxy-uridine or 5-methyleneaminouridine were introduced into 5S rRNA by T7 transcription. After incorporation of these uridine analogues into the transcript their amino groups were modified with 4-[3-(trifluoromethyl)-3 H -diazirin-3-yl]benzyl isothiocyanate or the N -hydroxysuccinimide ester of 4-[3-(trifluoromethyl)-3 H -diazirin-3-yl]benzoic acid respectively. 5S rRNA carrying the photoreactive diazirine groups (referred to as the 2'-aminoribose derivative and the 5-methyleneamino derivative respectively) was reconstituted into 50S subunits or 70S ribosomes. After mild UV irradiation cross-links formed to 23S rRNA were analysed by standard procedures. All of the observed cross-links involved residue U89 of the 5S rRNA. Three nucleotides of 23S rRNA were cross-linked to this residue with the 5-methyleneamino derivative, namely U958, G1022 and G1138. With the 2'-aminoribose derivative a single cross-link was found, to U958. The significance of these cross-links for our understanding of the structure and function of 5S rRNA and its environment in the ribosome are discussed.
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