ArticleBiophysical journal1998
Mechanical properties of actin filament networks depend on preparation, polymerization conditions, and storage of actin monomers.
Article in Biophysical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Roadmap to Local Tumour Growth: Insights from Cervical Cancer.Scientific reports · 2019Trial
- Actin Filament Pointed Ends: Assays for Regulation of Assembly and Disassembly by Tropomodulin and Tropomyosin.Cytoskeleton (Hoboken, N.J.) · 2025Article
- Branched actin cortices reconstituted in vesicles sense membrane curvature.Biophysical journal · 2023Article
- Micro-mechanical response and power-law exponents from the longitudinal fluctuations of F-actin solutions.Soft matter · 2023Article
- Review
- In vitro Preparation of Homogenous Actin Filaments for Dynamic and Electrophoretic Light Scattering Measurements.Bio-protocol · 2022Article
- Hydrodynamic and Polyelectrolyte Properties of Actin Filaments: Theory and Experiments.Polymers · 2022Article
- Theory of Weakly Polydisperse Cytoskeleton Filaments.Polymers · 2022Article
- The Cytoskeleton-A Complex Interacting Meshwork.Cells · 2019Review
- Glassy dynamics in composite biopolymer networks.Soft matter · 2018Article
- Emergent mechanics of actomyosin drive punctuated contractions and shape network morphology in the cell cortex.PLoS computational biology · 2018Article
- Actin filaments-A target for redox regulation.Cytoskeleton (Hoboken, N.J.) · 2016Review
- Life as a moving fluid: fate of cytoplasmic macromolecules in dynamic fungal syncytia.Current opinion in microbiology · 2015Review
- Actin Mechanics and Fragmentation.The Journal of biological chemistry · 2015Review
- Probing the mechanical properties of magnetosome chains in living magnetotactic bacteria.Nano letters · 2014Article
- Plasma membrane calcium ATPase activity is regulated by actin oligomers through direct interaction.The Journal of biological chemistry · 2013Article
- Nonlinear viscoelasticity of actin transiently cross-linked with mutant α-actinin-4.Journal of molecular biology · 2011Article
- Elasticity in ionically cross-linked neurofilament networks.Biophysical journal · 2010Article
- The proline-rich domain of tau plays a role in interactions with actin.BMC cell biology · 2009Article
- Computational analysis of viscoelastic properties of crosslinked actin networks.PLoS computational biology · 2009Article
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Authors and funding
6 authors.
Funding
Abstract
This study investigates possible sources for the variance of more than two orders of magnitude in the published values for the shear moduli of purified actin filaments. Two types of forced oscillatory rheometers used in some of our previous work agree within a factor of three for identical samples. Polymers assembled in EGTA and Mg2+ from fresh, gel-filtered ATP-actin at 1 mg/ml typically have an elastic storage modulus (G') of approximately 1 Pa at a deformation frequency of 0.1-1 Hz. G' is slightly higher when actin is polymerized in KCl with Ca2+ and Mg2+. Gel filtration removes minor contaminants from actin but has little effect on G' for most preparations of actin from acetone powder. Storage of actin monomers without frequent changes of buffer containing fresh ATP and dithiothreitol can result in changes that increase the G' of filaments by more than a factor of 10. Frozen storage can preserve the properties of monomeric actin, but care is necessary to prevent protein denaturation or aggregation due to freezing or thawing.
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